TY - JOUR
T1 - Effect of exercise on physiological age-related change at mouse neuromuscular junctions
AU - Alshuaib, Waleed B.
AU - Fahim, Mohamed A.
N1 - Funding Information:
The authors would like to thank Dr. A. A. Herrera for excellent advice, as well as Mrs. Jessica Maksaereekul and Mr. Michael Economos for unfailing technical assistance. This research was supported by United States Public Health Service Grant AG04755.
PY - 1990
Y1 - 1990
N2 - To determine the effect of endurance exercise on physiological age-related change at the mouse neuromuscular junction (NMJ), synaptic function was studied for extensor digitorum longus (EDL) and soleus muscles of three C57BL/6J mouse groups, 1) young adult control (YC: 10 months), 2) old control (OC: 20 months), and 3) old mice which exercised (OE: 20 months) since young-adulthood. Electrophysiological properties were studied with intracellular recording techniques. Safety margin was studied by measuring indirect isometric twitch tension in different calcium concentrations. With sedentary aging, EDL and soleus quantal contents increased. Following aging combined with 10 months of exercise, the EDL quantal contents in OE and YC animals were similar. In contrast, soleus quantal content was greater in OE than in YC animals. Determined safety margins were OC>YC=OE for EDL, and OC=YC=OE for soleus. This is the first study to indicate that physiological age-related changes at NMJs of EDL and soleus muscles are affected differently by endurance exercise. Exercise prevented all physiological age-related changes in EDL NMJs but not in soleus NMJs, this suggests that EDL changes are associated with inactivity during aging, while soleus changes are "fundamental" age changes.
AB - To determine the effect of endurance exercise on physiological age-related change at the mouse neuromuscular junction (NMJ), synaptic function was studied for extensor digitorum longus (EDL) and soleus muscles of three C57BL/6J mouse groups, 1) young adult control (YC: 10 months), 2) old control (OC: 20 months), and 3) old mice which exercised (OE: 20 months) since young-adulthood. Electrophysiological properties were studied with intracellular recording techniques. Safety margin was studied by measuring indirect isometric twitch tension in different calcium concentrations. With sedentary aging, EDL and soleus quantal contents increased. Following aging combined with 10 months of exercise, the EDL quantal contents in OE and YC animals were similar. In contrast, soleus quantal content was greater in OE than in YC animals. Determined safety margins were OC>YC=OE for EDL, and OC=YC=OE for soleus. This is the first study to indicate that physiological age-related changes at NMJs of EDL and soleus muscles are affected differently by endurance exercise. Exercise prevented all physiological age-related changes in EDL NMJs but not in soleus NMJs, this suggests that EDL changes are associated with inactivity during aging, while soleus changes are "fundamental" age changes.
KW - Aging
KW - Exercise
KW - Neuromuscular junction
KW - Synaptic plasticity
KW - Transmitter release
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U2 - 10.1016/0197-4580(90)90117-I
DO - 10.1016/0197-4580(90)90117-I
M3 - Article
C2 - 2234287
AN - SCOPUS:0025002476
SN - 0197-4580
VL - 11
SP - 555
EP - 561
JO - Neurobiology of Aging
JF - Neurobiology of Aging
IS - 5
ER -