Effect of lifestyle on age-related mitochondrial protein oxidation in mice cardiac muscle

Ana Isabel Padrão, Rita Ferreira, Rui Vitorino, Renato M.P. Alves, Pedro Figueiredo, José Alberto Duarte, Francisco Amado

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


This study investigated the influence of lifestyle on aging-related changes in cardiac proteins' oxidative modifications profile. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young Y, old sedentary S, and old active A). The S and A mice were individually placed into standard cages and in cages with running wheels, respectively, for 23 months. Upon killing, heart mitochondrial fractions were obtained for the evaluation of general proteins oxidative modifications profile, the identification of preferential protein targets, and oxidative phosphorylation (OXPHOS) activity. We observed age-related cardiac muscle impairment, evidenced by decreased OXPHOS activity, paralleled by an increased protein susceptibility to carbonylation and nitration. Among the main targets to these posttranslational modifications we found mitochondrial proteins, mainly from OXPHOS complexes, MnSOD and enzymes from lipid metabolism. Lifelong sedentary behavior exacerbated the nitrative damage of mitochondrial proteins, paralleled by a statistically significant decrease of respiratory chain complexes II and III activities. In overall, our results highlight the determinant role of aging in cardiac muscle impairment, which is worsened by a sedentary lifestyle.

Original languageEnglish
Pages (from-to)1467-1474
Number of pages8
JournalEuropean Journal of Applied Physiology
Issue number4
Publication statusPublished - Apr 2012
Externally publishedYes


  • Carbonylation
  • Mitochondria
  • Nitration
  • Oxidative phosphorylation
  • Wheel running

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)


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