Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati

Ikram-ul-Haq, Zhao Chang-Xing, Zahid Mukhtar, Cheruth Abdul Jaleel, Mohamed Mahgoub Azooz

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15 Citations (Scopus)


This experiment assessed the effect of partial physical desiccation on plant regeneration efficiency in scutellum-derived embryogenic calluses of rice (Oryza sativa L.) variety Super basmati. A number of callusing cultures were developed, and efficient callus induction was observed on MS (Murashige and Skoog) basal medium supplemented with 2.0 mg/L 2,4-dichlorophenoxy acetic acid. The calluses were proliferated on the same medium for 3 weeks and then shifted to dehydration desiccation treatment for 72 h. The desiccated calluses were cultured on different media for somatic embryogenesis and plant regeneration. A medium with 2.0 mg/L α-napthaleneacetic acid, 10.0 mg/L abscisic acid, 2.0 mg/L kinetin was best for somatic embryogenesis only, but not for further plant development. After 10 d, differentiated calluses were sub-cultured on medium with various concentrations and types of carbohydrates (carbon source) in 1MS2j medium. A large number of plantlets (14.51±2.81 and 8.56±2.90 plants/callus) were regenerated via chemical desiccation, on MS with 3% maltose+3% sorbitol and 6% sucrose, respectively. Under dehydration on only simple MS (3% sucrose), 11.23±3.22 plants/callus were developed. Under conditions of dehydration and chemical desiccation, plant regeneration rates were higher than the calluses cultured on simple MS medium in the presence of plant growth regulator. After somatic embryogenesis, >25% plants were sterile. The protocol used here may allow maximum regeneration of normal and fertile plantlets of super basmati rice within 3 months.

Original languageEnglish
Pages (from-to)1568-1575
Number of pages8
JournalJournal of Plant Physiology
Issue number14
Publication statusPublished - Sept 15 2009
Externally publishedYes


  • Physical desiccation
  • Plant regeneration efficiency
  • Relative callus proliferation rate
  • Somatic embryogenesis
  • Super basmati

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science


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