Effect of Piper betle on cardiac function, marker enzymes, and oxidative stress in isoproterenol-induced cardiotoxicity in rats

Dharamvir Singh Arya, Sachin Arora, Salma Malik, Saroj Nepal, Santosh Kumari, Shreesh Ojha

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


The present study was designed to investigate the cardioprotective potential of Piper betle (P. betle) against isoproterenol (ISP)-induced myocardial infarction in rats. Rats were randomly divided into eight groups viz. control, ISP, P. betle (75, 150, and 300mg/kg) and P. betle (75, 150, and 300mg/kg)+ ISP treated group. P. betle leaf extract (75, 150, or 300mg/kg) or saline was orally administered for 30 days. ISP (85mg/kg, s.c.) was administered at an interval of 24h on the 28th and 29th day and on day 30 the functional and biochemical parameters were measured. ISP administration showed a significant decrease in systolic, diastolic, mean arterial pressure (SAP, DAP, MAP), heart rate (HR), contractility (+LVdP/dt), and relaxation (-LVdP/dt) and increased left ventricular end-diastolic pressure (LVEDP). ISP also caused significant decrease in myocardial antioxidants; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), and myocyte injury marker enzymes; creatine phosphokinase-MB (CK-MB) isoenzyme and lactate dehydrogenase (LDH) along with enhanced lipid peroxidation; thiobarbituric acid reacting species (TBARS) in heart. Pre-treatment with P. betle favorably modulated hemodynamic (SAP, DAP, and MAP) and ventricular function parameters (-LVdP/dt and LVEDP). P. betle pre-treatment also restored SOD, CAT, GSH, and GPx, reduced the leakage of CK-MB isoenzyme and LDH along with decreased lipid peroxidation in the heart. Taken together, the biochemical and functional parameters indicate that P. betle 150 and 300mg/kg has a significant cardioprotective effect against ISP-induced myocardial infarction. Results of the present study suggest the cardioprotective potential of P. betle.

Original languageEnglish
Pages (from-to)564-571
Number of pages8
JournalToxicology Mechanisms and Methods
Issue number9
Publication statusPublished - Nov 2010
Externally publishedYes


  • Cardioprotection
  • Isoproterenol
  • Medicinal plants
  • Myocardial infarction.
  • Piper betle

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis


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