TY - JOUR
T1 - Effect of polystyrene particles on lung microvascular permeability in isolated perfused rabbit lungs
T2 - Role of size and surface properties
AU - Hamoir, J.
AU - Nemmar, A.
AU - Halloy, D.
AU - Wirth, D.
AU - Vincke, G.
AU - Vanderplasschen, A.
AU - Nemery, B.
AU - Gustin, P.
N1 - Funding Information:
This research was funded by the Fonds National de la Recherche Scientifique (FNRS) and the University of Liège (Belgium). Dr Julien Hamoir and Dr Alain Vanderplasschen are Research Fellow and Senior Research Associate of the FNRS, respectively. Purchase of the confocal microscope was supported by the following grants: FRFC 2.4532.98 from the FNRS, FNRS LOTTO 9.4592.97 from the Belgian National lottery, and ARC 98/03-220 from the French Community of Belgium.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The aim of this study was to investigate the role of particle number, total surface area, mass and surface chemical groups in (Kf,c) changes. The lung effects of four different fine (110 nm) and ultrafine (24 nm) polystyrene particles have been tested in an isolated perfused rabbit lung model. Pulmonary microvascular permeability (Kf,c) modifications were measured in response to intratracheal particle challenge. Polystyrene particles, mainly located in alveolar spaces and macrophages, induced a Kf,c increase that was related to the total surface area and number of particles rather than to the instilled mass. Moreover, the positively charged amine-modified polystyrene particles were more effective in the Kf,c response than the negatively charged carboxylate-modified polystyrene particles. We concluded that particle number and diameter that mathematically equally determined total surface area do not have the same importance in explaining the biological effects observed and that particle number could be an alternative to total surface area to describe the particle exposure. Furthermore, surface properties of polystyrene particles need to be considered to investigate the microvascular permeability changes measured in our model.
AB - The aim of this study was to investigate the role of particle number, total surface area, mass and surface chemical groups in (Kf,c) changes. The lung effects of four different fine (110 nm) and ultrafine (24 nm) polystyrene particles have been tested in an isolated perfused rabbit lung model. Pulmonary microvascular permeability (Kf,c) modifications were measured in response to intratracheal particle challenge. Polystyrene particles, mainly located in alveolar spaces and macrophages, induced a Kf,c increase that was related to the total surface area and number of particles rather than to the instilled mass. Moreover, the positively charged amine-modified polystyrene particles were more effective in the Kf,c response than the negatively charged carboxylate-modified polystyrene particles. We concluded that particle number and diameter that mathematically equally determined total surface area do not have the same importance in explaining the biological effects observed and that particle number could be an alternative to total surface area to describe the particle exposure. Furthermore, surface properties of polystyrene particles need to be considered to investigate the microvascular permeability changes measured in our model.
KW - Latex particles
KW - Lung
KW - Microvascular permeability
KW - Polystyrenes
KW - Ultrafine
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U2 - 10.1016/S0041-008X(03)00192-3
DO - 10.1016/S0041-008X(03)00192-3
M3 - Article
C2 - 12902199
AN - SCOPUS:0042593191
SN - 0041-008X
VL - 190
SP - 278
EP - 285
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
IS - 3
ER -