TY - JOUR
T1 - Effect of subcutaneous pancreatic tissue transplants on streptozotocin-induced diabetes in rats. II. Endocrine and metabolic functions
AU - Adeghate, E.
N1 - Funding Information:
The author wishes to thank Mr Abdul Samad Ponery and Shakur Abdulle for technical assistance. This work was supported by the Faculty of Medicine and Health Sciences, United Arab Emirates University (Research Grant number 95/22).
PY - 1999/2
Y1 - 1999/2
N2 - The present study examines the effect of subcutaneous pancreatic tissue grafts (SPTG) on endocrine and metabolic functions in streptozotocin (STZ)-induced diabetic rats using radioimmunoassay and biochemical techniques. SPTG survived even after 15 weeks of transplantation and significantly improved the weight of STZ-diabetic rats over a 15-week period. Although blood glucose-, cholesterol-, and glycosylated-haemoglobin (GHb) levels were not significantly lower in STZ-diabetic rats treated with SPTG, the values of these biochemical parameters were lower than those in untreated diabetic rats. Plasma and pancreatic immunoreactive C-peptide (IRCP) levels did not improve after SPTG (IRCP expressed as mean ± standard deviation were 0.22 ± 0.07, 0.072 ± 0.02 and 0.08 ± 0.03 pg ml-1 in the plasma non-diabetic, diabetic and treated rats respectively, while IRCP levels in the pancreas of the non-diabetic, diabetic and treated rats were 433.8 ± 0.1, 22.9 ± 0.01 and 10.4 ± 0.01 pg mg tissue-1 respectively). SPTG, however, improved plasma immunoreactive insulin (IRI) levels in both plasma and pancreas. IRI values in plasma were 54.7 ± 13.6, 18.0 ± 5.0 and 22.1 ± 4.3 μUI ml-1 in non-diabetic, diabetic and treated rats respectively and were 277.3 ± 37.1, 14.7 ± 1.8 and 30.3 ± 15.9 μIU μg tissue-1 in the pancreas of non-diabetic, diabetic and treated rats respectively. There was improvement in immunoreactive glucagon (IRG) levels after SPTG. IRG values in the plasma of non-diabetic, diabetic and treated rats were 147.0 ± 10.7, 408.0 ± 76.5 and 247.7 ± 3 pg ml-1 respectively whereas, IRG measured in the pancreas was 1642.25 ± 424.23, 1899.0 ± 290.4 and 1714.1 ± 301.98 pg μg tissue-1 in non-diabetic, diabetic and treated rats, respectively. The pancreas:plasma ratio of pancreatic hormones was deranged in untreated diabetes but improved after SPTG, In conclusion, SPTG significantly improved the weight gain, pancreatic insulin content, plasma IRG and pancreas: plasma ratio of IRCP, IRI and IRG. It also reduced blood glucose-, cholesterol-, and glycosylated-hemoglobin levels in STZ-diabetic rats.
AB - The present study examines the effect of subcutaneous pancreatic tissue grafts (SPTG) on endocrine and metabolic functions in streptozotocin (STZ)-induced diabetic rats using radioimmunoassay and biochemical techniques. SPTG survived even after 15 weeks of transplantation and significantly improved the weight of STZ-diabetic rats over a 15-week period. Although blood glucose-, cholesterol-, and glycosylated-haemoglobin (GHb) levels were not significantly lower in STZ-diabetic rats treated with SPTG, the values of these biochemical parameters were lower than those in untreated diabetic rats. Plasma and pancreatic immunoreactive C-peptide (IRCP) levels did not improve after SPTG (IRCP expressed as mean ± standard deviation were 0.22 ± 0.07, 0.072 ± 0.02 and 0.08 ± 0.03 pg ml-1 in the plasma non-diabetic, diabetic and treated rats respectively, while IRCP levels in the pancreas of the non-diabetic, diabetic and treated rats were 433.8 ± 0.1, 22.9 ± 0.01 and 10.4 ± 0.01 pg mg tissue-1 respectively). SPTG, however, improved plasma immunoreactive insulin (IRI) levels in both plasma and pancreas. IRI values in plasma were 54.7 ± 13.6, 18.0 ± 5.0 and 22.1 ± 4.3 μUI ml-1 in non-diabetic, diabetic and treated rats respectively and were 277.3 ± 37.1, 14.7 ± 1.8 and 30.3 ± 15.9 μIU μg tissue-1 in the pancreas of non-diabetic, diabetic and treated rats respectively. There was improvement in immunoreactive glucagon (IRG) levels after SPTG. IRG values in the plasma of non-diabetic, diabetic and treated rats were 147.0 ± 10.7, 408.0 ± 76.5 and 247.7 ± 3 pg ml-1 respectively whereas, IRG measured in the pancreas was 1642.25 ± 424.23, 1899.0 ± 290.4 and 1714.1 ± 301.98 pg μg tissue-1 in non-diabetic, diabetic and treated rats, respectively. The pancreas:plasma ratio of pancreatic hormones was deranged in untreated diabetes but improved after SPTG, In conclusion, SPTG significantly improved the weight gain, pancreatic insulin content, plasma IRG and pancreas: plasma ratio of IRCP, IRI and IRG. It also reduced blood glucose-, cholesterol-, and glycosylated-hemoglobin levels in STZ-diabetic rats.
KW - Cholesterol
KW - Glycosylated haemoglobin
KW - Pancreatic hormones
KW - Radioimmunoassay
KW - Rats
KW - Streptozotocin-induced diabetes
KW - Total protein
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U2 - 10.1054/tice.1999.0007
DO - 10.1054/tice.1999.0007
M3 - Article
C2 - 10368988
AN - SCOPUS:0033081991
SN - 0040-8166
VL - 31
SP - 73
EP - 83
JO - Tissue and Cell
JF - Tissue and Cell
IS - 1
ER -