TY - JOUR
T1 - Effects of Nanosilica Priming on Rapeseed (Brassica napus) Tolerance to Cadmium and Arsenic Stress by Regulating Cellular Metabolism and Antioxidant Defense
AU - Ulhassan, Zaid
AU - Ali, Sharafat
AU - Kaleem, Zohaib
AU - Shahbaz, Hafsah
AU - He, Di
AU - Khan, Ali Raza
AU - Salam, Abdul
AU - Hamid, Yasir
AU - Sheteiwy, Mohamed S.
AU - Zhou, Weijun
AU - Huang, Qian
N1 - Publisher Copyright:
© 2025 American Chemical Society.
PY - 2025/2/26
Y1 - 2025/2/26
N2 - The mechanisms by which seed-primed silicon dioxide nanoparticles (nSi) alleviated arsenic (As) and cadmium (Cd) toxicity in Brassica napus L. remain unclear. A pot study examined the physico-biochemical, cellular, and molecular responses of B. napus exposed to Cd (10 mg/kg soil) and As (50 mg/kg soil) doses with or without nSi priming. The results showed that nSi priming improved photosynthesis, seedling biomass, and metabolite accumulation, and restored the cell structure. Upon Cd and As stress, nSi diminished oxidative stress by downplaying H2O2 (24-32%) and O2•- (29-36%), MDA, and activating antioxidant defenses. Also, nSi relieved Cd and As accumulation (27-36%) by enhancing root-vacuolar sequestration (upregulating BnHMA3, BnPCs, and BnABCC1), cell wall chelation, and downregulating root transporters (BnNRAMP5, BnIRTI, BnHMA2, BnHMA4, BnPHT1.1, and BnPHT1.4). Our findings revealed that nSi priming effectively enhanced canola tolerance to Cd and As toxicity by strengthening multiple oxidative defense mechanisms and limiting their accumulation.
AB - The mechanisms by which seed-primed silicon dioxide nanoparticles (nSi) alleviated arsenic (As) and cadmium (Cd) toxicity in Brassica napus L. remain unclear. A pot study examined the physico-biochemical, cellular, and molecular responses of B. napus exposed to Cd (10 mg/kg soil) and As (50 mg/kg soil) doses with or without nSi priming. The results showed that nSi priming improved photosynthesis, seedling biomass, and metabolite accumulation, and restored the cell structure. Upon Cd and As stress, nSi diminished oxidative stress by downplaying H2O2 (24-32%) and O2•- (29-36%), MDA, and activating antioxidant defenses. Also, nSi relieved Cd and As accumulation (27-36%) by enhancing root-vacuolar sequestration (upregulating BnHMA3, BnPCs, and BnABCC1), cell wall chelation, and downregulating root transporters (BnNRAMP5, BnIRTI, BnHMA2, BnHMA4, BnPHT1.1, and BnPHT1.4). Our findings revealed that nSi priming effectively enhanced canola tolerance to Cd and As toxicity by strengthening multiple oxidative defense mechanisms and limiting their accumulation.
KW - Brassica napus L
KW - heavy metals
KW - nanotechnology
KW - oxidative stress
KW - silicon nanoparticles
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U2 - 10.1021/acs.jafc.4c08246
DO - 10.1021/acs.jafc.4c08246
M3 - Article
C2 - 39937631
AN - SCOPUS:85214696102
SN - 0021-8561
VL - 73
SP - 4518
EP - 4533
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 8
ER -