Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat

Ahmed Sultan, Ernest Adeghate, Bright Starling Emerald, Muhammad A. Qureshi, Saeed Tariq Minhas, Frank Christopher Howarth

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

(1) Background: Cardiovascular complications are a leading cause of morbidity and mortality in diabetic patients. The effects of obesity and diabesity on the function and structure of ventricular myocytes in the Zucker fatty (ZF) rat and the Zucker diabetic fatty (ZDF) rat compared to Zucker lean (ZL) control rats have been investigated. (2) Methods: Shortening and intracellular Ca2+ were simultaneously measured with cell imaging and fluorescence photometry, respectively. Ventricular muscle protein expression and structure were investigated with Western blot and electron microscopy, respectively. (3) Results: The amplitude of shortening was increased in ZF compared to ZL but not compared to ZDF myocytes. Resting Ca2+ was increased in ZDF compared to ZL myocytes. Time to half decay of the Ca2+ transient was prolonged in ZDF compared to ZL and was reduced in ZF compared to ZL myocytes. Changes in expression of proteins associated with cardiac muscle contraction are presented. Structurally, there were reductions in sarcomere length in ZDF and ZF compared to ZL and reductions in mitochondria count in ZF compared to ZDF and ZL myocytes. (4) Conclusions: Alterations in ventricular muscle proteins and structure may partly underlie the defects observed in Ca2+ signaling in ZDF and ZF compared to ZL rat hearts.

Original languageEnglish
Article number1221
JournalLife
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 2022

Keywords

  • Zucker diabetic fatty rat
  • Zucker fatty rat
  • Zucker lean rat
  • heart
  • intracellular Ca
  • mitochondria
  • sarcomere
  • shortening
  • ventricular muscle structure
  • ventricular myocytes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Space and Planetary Science
  • Palaeontology

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