Effects of streptozotocin-induced long-term diabetes on parietal cell function and morphology in rats

Salim M.A. Bastaki, Ernest Adeghate, Irwin S. Chandranath, Naheed Amir, Saeed Tariq, Rashed S. Hameed, Abdu Adem

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14 Citations (Scopus)


Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats by a single intraperitoneal injection of streptozotocin (60 mg/kg body weight). The rats were weighed weekly and sacrificed after 6 months. The glandular portion of the stomach was removed and processed for H +-K +-ATPase immunohistochemistry and light and electron microscopy studies. Acid secretion was measured in vivo. After 6 months of diabetes, the mean weight of the rats was significantly lower (P < 0.001) compared to control. The mean weight of the stomach to body weight percentage increased significantly (P < 0.001) compared to control. The blood glucose level in diabetic rats was significantly higher (P < 0.001) than in normal control. Diabetic rats showed significant (P < 0.001) decrease in basal and stimulated acid secretion when compared to control. Electron micrographs of the parietal cells of glandular stomach of diabetic rats revealed significant (P < 0.0002) reduction in the number of mitochondria and a small though not significant increase in the number of canaliculi in the parietal cells compared with normal. Immunohistochemistry showed reduced H +-K +-ATPase (P < 0.00001) compared to control. Long-term diabetes induces morphological as well as functional changes in gastric parietal cells. The decrease in the number of mitochondria accompanied by reduced in H +-K +-ATPase in parietal cells may explain the reduced acid secretion observed in diabetics.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalMolecular and cellular biochemistry
Issue number1-2
Publication statusPublished - Aug 2010


  • Acid secretion
  • Diabetes mellitus
  • H , K -ATPase
  • Mitochondria
  • Parietal cells

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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