TY - JOUR
T1 - Efficacy of six frog skin-derived antimicrobial peptides against colistin-resistant strains of the Acinetobacter baumannii group
AU - Conlon, J. Michael
AU - Sonnevend, Agnes
AU - Pál, Tibor
AU - Vila-Farrés, Xavier
N1 - Funding Information:
Funding : This work was supported by a Faculty Support Grant ( NP-10-11/103 ) and a University Research Grant from the United Arab Emirates University (Al-Ain, United Arab Emirates).
PY - 2012/4
Y1 - 2012/4
N2 - The emergence of Acinetobacter sp. strains resistant to all antibacterial agents including colistin necessitates the development of new types of antimicrobial agents. Six cationic α-helical frog skin-derived peptides (CPF-AM1, PGLa-AM1, B2RP-ERa, [E4K]alyteserin-1c, [D4K]B2RP and [G4K]XT-7) were selected for this study on the basis of potent growth-inhibitory activity against Gram-negative bacteria and low haemolytic activity against human erythrocytes. All peptides were active against a range of colistin-susceptible [minimum inhibitory concentration (MIC) ≤ 2 μg/mL] and colistin-resistant (MIC ≥ 64 μg/mL) clinical isolates of multidrug-resistant strains of Acinetobacter baumannii and Acinetobacter nosocomialis. The most potent peptides against the colistin-resistant strains were [D4K]B2RP and [E4K]alyteserin-1c (MIC = 4-16 μg/mL for both). The MIC values of these peptides against the colistin-susceptible strains were in the same range. The frog peptides show potential for development into drugs to treat infections caused by pandrug-resistant Gram-negative pathogens.
AB - The emergence of Acinetobacter sp. strains resistant to all antibacterial agents including colistin necessitates the development of new types of antimicrobial agents. Six cationic α-helical frog skin-derived peptides (CPF-AM1, PGLa-AM1, B2RP-ERa, [E4K]alyteserin-1c, [D4K]B2RP and [G4K]XT-7) were selected for this study on the basis of potent growth-inhibitory activity against Gram-negative bacteria and low haemolytic activity against human erythrocytes. All peptides were active against a range of colistin-susceptible [minimum inhibitory concentration (MIC) ≤ 2 μg/mL] and colistin-resistant (MIC ≥ 64 μg/mL) clinical isolates of multidrug-resistant strains of Acinetobacter baumannii and Acinetobacter nosocomialis. The most potent peptides against the colistin-resistant strains were [D4K]B2RP and [E4K]alyteserin-1c (MIC = 4-16 μg/mL for both). The MIC values of these peptides against the colistin-susceptible strains were in the same range. The frog peptides show potential for development into drugs to treat infections caused by pandrug-resistant Gram-negative pathogens.
KW - Acinetobacter baumannii
KW - Antimicrobial peptide
KW - Colistin resistance
KW - Frog skin
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U2 - 10.1016/j.ijantimicag.2011.12.005
DO - 10.1016/j.ijantimicag.2011.12.005
M3 - Article
C2 - 22326566
AN - SCOPUS:84858278345
SN - 0924-8579
VL - 39
SP - 317
EP - 320
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 4
ER -