TY - JOUR
T1 - Enhancement of mucosal immune response against HIV-1 Gag by DNA immunization
AU - Yoshizawa, Izumi
AU - Soda, Yoko
AU - Mizuochi, Toshiaki
AU - Yasuda, Sachiko
AU - Rizvi, Tahir A.
AU - Mizuochi, Tsuguo
AU - Takemori, Toshitada
AU - Tsunetsugu-Yokota, Yasuko
N1 - Funding Information:
We thank Mrs Akiko Kurata for her excellent technical help. This work was supported by grants from the Ministry of Public Health and Welfare of Japan and partly from the Human Science Foundation of Japan. I.Y. is a post-doctoral fellow supported by the Japanese Foundation for AIDS Prevention.
PY - 2001/4/6
Y1 - 2001/4/6
N2 - In order to examine the feasibility of Gag-expression DNA as a potential candidate for HIV vaccine using a mouse model, we injected DNA into mice either intramuscularly or by using a gene gun. Both methods induced a low level of antibody production. However, after booster immunization with p24 protein emulsified with complete Freund's adjuvant via a footpad, we found that only the preceding intramuscular DNA immunization induced an anti-Gag Th1-type (IgG2a) antibody response, in addition to the enhancement of a Th2-type (IgG1) antibody response. Importantly, when mice were boosted intranasally with p24 and cholera toxin, intramuscular DNA injection was found to enhance both systemic and mucosal Gag-specific immune responses. These results indicate that intramuscular DNA immunization confers the inducibility of memory cells, which circulate around various mucosal tissues. Therefore, intramuscular DNA priming, followed by a mucosal booster immunization, could be considered as a regimen applicable to HIV vaccine.
AB - In order to examine the feasibility of Gag-expression DNA as a potential candidate for HIV vaccine using a mouse model, we injected DNA into mice either intramuscularly or by using a gene gun. Both methods induced a low level of antibody production. However, after booster immunization with p24 protein emulsified with complete Freund's adjuvant via a footpad, we found that only the preceding intramuscular DNA immunization induced an anti-Gag Th1-type (IgG2a) antibody response, in addition to the enhancement of a Th2-type (IgG1) antibody response. Importantly, when mice were boosted intranasally with p24 and cholera toxin, intramuscular DNA injection was found to enhance both systemic and mucosal Gag-specific immune responses. These results indicate that intramuscular DNA immunization confers the inducibility of memory cells, which circulate around various mucosal tissues. Therefore, intramuscular DNA priming, followed by a mucosal booster immunization, could be considered as a regimen applicable to HIV vaccine.
KW - DNA immunization
KW - HIV-1 Gag
KW - Mucosal immune response
UR - http://www.scopus.com/inward/record.url?scp=0035815542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035815542&partnerID=8YFLogxK
U2 - 10.1016/S0264-410X(00)00539-9
DO - 10.1016/S0264-410X(00)00539-9
M3 - Article
C2 - 11282211
AN - SCOPUS:0035815542
SN - 0264-410X
VL - 19
SP - 2995
EP - 3003
JO - Vaccine
JF - Vaccine
IS - 20-22
ER -