TY - JOUR
T1 - Enzymatic biodiesel production of microalgae lipids under supercritical carbon dioxide
T2 - Process optimization and integration
AU - Taher, Hanifa
AU - Al-Zuhair, Sulaiman
AU - Al-Marzouqi, Ali H.
AU - Haik, Yousef
AU - Farid, Mohammed
PY - 2014
Y1 - 2014
N2 - Enzymatic biodiesel production in supercritical CO2 (SC-CO2) has recently received an increasing attention, as an alternative to the conventional chemical processes. In this study, enzymatic production of biodiesel from microalgal lipids was investigated in batch and integrated extraction-reaction systems. In the batch system, the effect of enzyme loading (15-50 wt%), temperature (35-55°C) and methanol to lipid molar ratios (3-15:1) were studied, and response surface methodology was employed to optimize selected factors effect. The optimum transesterification yield of 80% was obtained at 47°C, 200 bar, 35% enzyme loading, and 9:1 molar ratio after 4 h reaction in the batch system. The experimental results were also used to determine the kinetics parameters of the Ping-Pong Bi Bi model, with methanol inhibition, suggested to describe the reaction. In the continuous integrated extraction-reaction system, the effect of methanol to lipids molar ratio was investigated, and enzyme operational stability and reusability were tested. Bed regeneration by tert-butanol washing was also assessed. The optimum methanol to lipid ratio was found to be 10:1. At this ratio, the enzyme was able to attain 78% of its original activity when reused for 6 continuous cycles, and the bed was successfully reused by washing with tert-butanol.
AB - Enzymatic biodiesel production in supercritical CO2 (SC-CO2) has recently received an increasing attention, as an alternative to the conventional chemical processes. In this study, enzymatic production of biodiesel from microalgal lipids was investigated in batch and integrated extraction-reaction systems. In the batch system, the effect of enzyme loading (15-50 wt%), temperature (35-55°C) and methanol to lipid molar ratios (3-15:1) were studied, and response surface methodology was employed to optimize selected factors effect. The optimum transesterification yield of 80% was obtained at 47°C, 200 bar, 35% enzyme loading, and 9:1 molar ratio after 4 h reaction in the batch system. The experimental results were also used to determine the kinetics parameters of the Ping-Pong Bi Bi model, with methanol inhibition, suggested to describe the reaction. In the continuous integrated extraction-reaction system, the effect of methanol to lipids molar ratio was investigated, and enzyme operational stability and reusability were tested. Bed regeneration by tert-butanol washing was also assessed. The optimum methanol to lipid ratio was found to be 10:1. At this ratio, the enzyme was able to attain 78% of its original activity when reused for 6 continuous cycles, and the bed was successfully reused by washing with tert-butanol.
KW - Biodiesel
KW - Lipase
KW - Microalgae lipid
KW - Process integration
KW - Production kinetics
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U2 - 10.1016/j.bej.2014.05.019 Regular article
DO - 10.1016/j.bej.2014.05.019 Regular article
M3 - Article
SN - 1369-703X
VL - 90
SP - 103
EP - 113
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
ER -