Epstein-Barr virus infection correlates with the expression of COX-2, p16INK4A and p53 in classic Hodgkin lymphoma

Suhail Al-Salam, Aktham Awwad, Manjusha Sudhadevi, Sayel Daoud, Nico J.D. Nagelkerke, Antonio Castella, S. M. Chong, Mouied Alashari

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Classic Hodgkin lymphoma (cHL), a germinal-center related B cell neoplasm in almost all cases, is characterized by scarcity of the neoplastic Hodgkin/Reed-Sternberg (H/RS) cells. Epstein-Barr virus (EBV) has been shown to affect cell cycle and regulation of apoptosis. In total, 95 cases of cHL were studied. Five-micrometer sections were prepared and stained with hematoxylin and eosin and immunohistochemical streptavidin-biotin methods for EBV-LMP-1, COX-2, p53, p16, ki-67 and cleaved caspase-3. In-situ hybridization for EBV encoded RNA was used to confirm the detection of EBV in H/RS. There were 49 nodular sclerosis, 32 mixed cellularity, 8 lymphocyte-rich, and 6 lymphocyte-depleted subtypes in this series of cases. EBV, COX-2, p16INK4A and p53 were detected in 55% (52/95), 64% (61/95), 62% (59/95), and 65% (62/95) of the cases respectively. EBV was detected in 62% (38/61), 70% (41/59), and 69% (43/62) of COX2, p16 and p53 positive cases respectively. On the other hand, EBV-non-infected cases of cHL are associated with 59% (20/34), 69% (25/36), and 73% (24/33) of COX2, p16 and p53 negative cases respectively. In conclusion, EBV infection is associated with the expression of COX-2, p16INK4A and p53. EBV might be the dominant factor in determining the expression of these three proteins.

Original languageEnglish
Pages (from-to)2765-2777
Number of pages13
JournalInternational Journal of Clinical and Experimental Pathology
Issue number12
Publication statusPublished - 2013


  • COX-2
  • Epstein Barr virus
  • Hodgkin lymphoma
  • P16
  • P53

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology


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