Epstein-Barr virus infection is inversely correlated with the expression of retinoblastoma protein in reed-sternberg cells in classic Hodgkin lymphoma

Suhail Al-Salam, Aktham Awwad, Mouied Alashari

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Classic Hodgkin lymphoma (cHL) is characterized by few neoplastic Hodgkin/Reed-Sternberg (H/RS) cells in a background of intense inflammatory infiltrate. Epstein-Barr virus (EBV) has been shown to affect cell cycle and regulation of apoptosis. In total, 82 cases of cHL were studied. Five- micrometer sections were prepared and stained with haematoxylin and eosin and immunohistochemical streptavidin-biotin methods for EBV-LMP-1, pRb, ki-67 and cleaved caspase-3. In-situ hybridization for EBV encoded RNA was used to confirm the detection of EBV in H/RS cells. There were 45 nodular sclerosis, 28 mixed cellularity, 4 lymphocyte-rich, and 5 lymphocyte depletion subtypes in this series of cases. EBV and pRb were detected in 55% (46/82) and 64% (50/82) of the cases respectively. EBV was detected in 78% (25/32) of pRb-negative cases and 81% (29/36) of EBV-negative cases are pRb-positive. A statistically significant inverse relationship was observed between the presence of EBV and expression of pRb (P = 0.001). In conclusion, EBV infection is inversely correlated with pRb in H/RS cells in cHL.

Original languageEnglish
Pages (from-to)7508-7517
Number of pages10
JournalInternational Journal of Clinical and Experimental Pathology
Volume7
Issue number11
Publication statusPublished - 2014

Keywords

  • Epstein barr virus
  • Hodgkin lymphoma
  • PRb

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Fingerprint

Dive into the research topics of 'Epstein-Barr virus infection is inversely correlated with the expression of retinoblastoma protein in reed-sternberg cells in classic Hodgkin lymphoma'. Together they form a unique fingerprint.

Cite this