TY - JOUR
T1 - Establishment of tumor cell culture (ILA) derived from hamster pancreatic islets treated with bop
AU - Toshkov, Ilia
AU - Schmied, Bruno
AU - Adrian, Thomas E.
AU - Murphy, Leo
AU - Haay, Wahab
AU - Pour, Parviz M.
PY - 1998
Y1 - 1998
N2 - ILA cells were established from tumors induced by the pancreatic carcinogen N-nitrosobis(2-oxopropyl)amine (BOP) in hamster islets. The proliferation, morphology, karyotype, immunoreactivity with certain antibodies and growth factor secretion of these tumor cells were compared with the same parameters in tumor cells induced by BOP in hamster ductal cells (TAKA-I-BOP) established in a previous study. Minor differences were found in the morphology and ultrastructure of the 2 cell lines. Contrary to TAKA-I-BOP cells, ILA cells did not express cytokeratins 8.13, 13 or 18 but did express DU-PAN-2 and TAG-72, 2 known human pancreatic cancer-associated antigens. No endocrine cell markers were expressed. A significant difference also was found in the chromosomal pattern in that there were more abnormalities and marker chromosomes in ILA cells than in TAKA-I-BOP cells and the Y or X chromosomes were missing in ILA cells. ILA cells produced TGF- α, IGF-I, bombesin and gastrin and expressed specific binding sites for hEGF. TGF-α secretion from ILA cells was much greater than that from TAKA- I-BOP cells. Our results indicate that pancreatic cancer cells grown in vitro are not a single clone. We conclude that there are some genetic and biological differences between tumors arising from pancreatic duct and islets and that pancreatic ductal adenocarcinomas originating from islets have a profound malignant potential.
AB - ILA cells were established from tumors induced by the pancreatic carcinogen N-nitrosobis(2-oxopropyl)amine (BOP) in hamster islets. The proliferation, morphology, karyotype, immunoreactivity with certain antibodies and growth factor secretion of these tumor cells were compared with the same parameters in tumor cells induced by BOP in hamster ductal cells (TAKA-I-BOP) established in a previous study. Minor differences were found in the morphology and ultrastructure of the 2 cell lines. Contrary to TAKA-I-BOP cells, ILA cells did not express cytokeratins 8.13, 13 or 18 but did express DU-PAN-2 and TAG-72, 2 known human pancreatic cancer-associated antigens. No endocrine cell markers were expressed. A significant difference also was found in the chromosomal pattern in that there were more abnormalities and marker chromosomes in ILA cells than in TAKA-I-BOP cells and the Y or X chromosomes were missing in ILA cells. ILA cells produced TGF- α, IGF-I, bombesin and gastrin and expressed specific binding sites for hEGF. TGF-α secretion from ILA cells was much greater than that from TAKA- I-BOP cells. Our results indicate that pancreatic cancer cells grown in vitro are not a single clone. We conclude that there are some genetic and biological differences between tumors arising from pancreatic duct and islets and that pancreatic ductal adenocarcinomas originating from islets have a profound malignant potential.
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U2 - 10.1002/(SICI)1097-0215(19981123)78:5<636::AID-IJC18>3.0.CO;2-5
DO - 10.1002/(SICI)1097-0215(19981123)78:5<636::AID-IJC18>3.0.CO;2-5
M3 - Article
C2 - 9808535
AN - SCOPUS:0031792369
SN - 0020-7136
VL - 78
SP - 636
EP - 641
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 5
ER -