Examination of the failure properties of the anterior cruciate ligament during the estrous cycle

M. D. Rau; D. Renouf; D. Benfield; D. D. Otto; G. M. Thornton; V. J. Raso; K. M. Bagnall

doi:10.1016/j.knee.2003.12.003

Examination of the failure properties of the anterior cruciate ligament during the estrous cycle

M. D. Rau, D. Renouf, D. Benfield, D. D. Otto, G. M. Thornton, V. J. Raso, K. M. Bagnall

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The purpose of this study was to determine whether the mechanical properties of the rat anterior cruciate ligament (ACL) vary when tested in vitro at different stages of the estrous cycle. Sixty female rats were allocated to four groups according to their stage of the estrous cycle: diestrus (n=16), proestrus (n=17), estrus (n=13) and metestrus (n=14). Right hindlimbs were harvested for mechanical testing and left hindlimbs were harvested for immunohistochemical staining to confirm the presence of the estrogen receptor. Results from the first relaxation test showed a significant difference between the estrus and proestrus stage, which was not observed in a second subsequent relaxation test. Likewise, no significant differences were found when comparing failure load and stiffness between the different stages of the estrous cycle. These results suggest that normal physiological fluctuations in estrogen during the estrous cycle did not alter the failure properties of the rat ACL.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Knee
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/j.knee.2003.12.003
Publication status	Published - Jan 2005
Externally published	Yes

Keywords

Anterior cruciate ligament
Failure properties
Hormonal cycle
Immunohistochemistry
Mechanical testing

ASJC Scopus subject areas

Orthopedics and Sports Medicine

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10.1016/j.knee.2003.12.003

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title = "Examination of the failure properties of the anterior cruciate ligament during the estrous cycle",

abstract = "The purpose of this study was to determine whether the mechanical properties of the rat anterior cruciate ligament (ACL) vary when tested in vitro at different stages of the estrous cycle. Sixty female rats were allocated to four groups according to their stage of the estrous cycle: diestrus (n=16), proestrus (n=17), estrus (n=13) and metestrus (n=14). Right hindlimbs were harvested for mechanical testing and left hindlimbs were harvested for immunohistochemical staining to confirm the presence of the estrogen receptor. Results from the first relaxation test showed a significant difference between the estrus and proestrus stage, which was not observed in a second subsequent relaxation test. Likewise, no significant differences were found when comparing failure load and stiffness between the different stages of the estrous cycle. These results suggest that normal physiological fluctuations in estrogen during the estrous cycle did not alter the failure properties of the rat ACL.",

keywords = "Anterior cruciate ligament, Failure properties, Hormonal cycle, Immunohistochemistry, Mechanical testing",

author = "Rau, {M. D.} and D. Renouf and D. Benfield and Otto, {D. D.} and Thornton, {G. M.} and Raso, {V. J.} and Bagnall, {K. M.}",

note = "Funding Information: The authors gratefully acknowledge the technical assistance of D.J. Raso and the financial support of the Edmonton Orthopaedic Research Committee and the Natural Sciences and Engineering Research Council of Canada (NSERC). ",

year = "2005",

month = jan,

doi = "10.1016/j.knee.2003.12.003",

language = "English",

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AU - Rau, M. D.

AU - Renouf, D.

AU - Benfield, D.

AU - Otto, D. D.

AU - Thornton, G. M.

AU - Raso, V. J.

AU - Bagnall, K. M.

N1 - Funding Information: The authors gratefully acknowledge the technical assistance of D.J. Raso and the financial support of the Edmonton Orthopaedic Research Committee and the Natural Sciences and Engineering Research Council of Canada (NSERC).

PY - 2005/1

Y1 - 2005/1

N2 - The purpose of this study was to determine whether the mechanical properties of the rat anterior cruciate ligament (ACL) vary when tested in vitro at different stages of the estrous cycle. Sixty female rats were allocated to four groups according to their stage of the estrous cycle: diestrus (n=16), proestrus (n=17), estrus (n=13) and metestrus (n=14). Right hindlimbs were harvested for mechanical testing and left hindlimbs were harvested for immunohistochemical staining to confirm the presence of the estrogen receptor. Results from the first relaxation test showed a significant difference between the estrus and proestrus stage, which was not observed in a second subsequent relaxation test. Likewise, no significant differences were found when comparing failure load and stiffness between the different stages of the estrous cycle. These results suggest that normal physiological fluctuations in estrogen during the estrous cycle did not alter the failure properties of the rat ACL.

AB - The purpose of this study was to determine whether the mechanical properties of the rat anterior cruciate ligament (ACL) vary when tested in vitro at different stages of the estrous cycle. Sixty female rats were allocated to four groups according to their stage of the estrous cycle: diestrus (n=16), proestrus (n=17), estrus (n=13) and metestrus (n=14). Right hindlimbs were harvested for mechanical testing and left hindlimbs were harvested for immunohistochemical staining to confirm the presence of the estrogen receptor. Results from the first relaxation test showed a significant difference between the estrus and proestrus stage, which was not observed in a second subsequent relaxation test. Likewise, no significant differences were found when comparing failure load and stiffness between the different stages of the estrous cycle. These results suggest that normal physiological fluctuations in estrogen during the estrous cycle did not alter the failure properties of the rat ACL.

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KW - Failure properties

KW - Hormonal cycle

KW - Immunohistochemistry

KW - Mechanical testing

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Examination of the failure properties of the anterior cruciate ligament during the estrous cycle

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Keywords

ASJC Scopus subject areas

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