Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH

Mutamed Ayyash; Constantinos Stathopoulos; Basim Abu-Jdayil; Gennaro Esposito; Mohammad Baig; Mark S. Turner; Ahmad Salihin Baba; Vasso Apostolopoulos; Anas Al-Nabulsi; Tareq Osaili

doi:10.1016/j.lwt.2020.109741

Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH

Mutamed Ayyash, Constantinos Stathopoulos, Basim Abu-Jdayil, Gennaro Esposito, Mohammad Baig, Mark S. Turner, Ahmad Salihin Baba, Vasso Apostolopoulos, Anas Al-Nabulsi, Tareq Osaili

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21 Citations (Scopus)

Abstract

Microbial exopolysaccharide (EPS) is of interest due to their applications in food processing and potential health benefits following consumption. The objectives of this study were to: 1) isolate, purify and characterize EPS produced by Enterococcus faecium MS79 (EPS-MS79); 2) investigate bioactive properties of EPS-MS79; and 3) investigate the rheological properties of the EPS-MS79 as influenced by salt type and pH level. The average molecular weight of the EPS-MS79 was 8.3 × 10⁵ Da and it is composed of the three monosaccharides arabinose, mannose, and glucose in a molar ratio of 0.8:1.7:11.3. Scavenging activities of EPS-MS79 were 76% and 85% as measured by DPPH and 26% and 44% as measured by ABTS. The antiproliferative activities against colon (72% and 77%) and breast (43% and 56%) cancer cell lines. EPS-MS79 inhibited the growth of bacterial pathogens by up to 3 logs compared to the control. All EPS-MS79 solutions displayed shear thinning behavior. The storage and loss moduli of all EPS-MS79 solutions increased alongside with the frequency increase. Salt type and pH level impacted on the rheological properties of EPS-MS79. The EPS-MS79 could be a potent prebiotic promoting health and rheological properties of foods.

Original language	English
Article number	109741
Journal	LWT
Volume	131
DOIs	https://doi.org/10.1016/j.lwt.2020.109741
Publication status	Published - Sept 2020

Keywords

Anticancer
Antidiabetic
Apparent viscosity
Probiotic
Thixotropic

ASJC Scopus subject areas

Food Science

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10.1016/j.lwt.2020.109741

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Ayyash, M., Stathopoulos, C., Abu-Jdayil, B., Esposito, G., Baig, M., Turner, M. S., Baba, A. S., Apostolopoulos, V., Al-Nabulsi, A., & Osaili, T. (2020). Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH. LWT, 131, Article 109741. https://doi.org/10.1016/j.lwt.2020.109741

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title = "Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH",

abstract = "Microbial exopolysaccharide (EPS) is of interest due to their applications in food processing and potential health benefits following consumption. The objectives of this study were to: 1) isolate, purify and characterize EPS produced by Enterococcus faecium MS79 (EPS-MS79); 2) investigate bioactive properties of EPS-MS79; and 3) investigate the rheological properties of the EPS-MS79 as influenced by salt type and pH level. The average molecular weight of the EPS-MS79 was 8.3 × 105 Da and it is composed of the three monosaccharides arabinose, mannose, and glucose in a molar ratio of 0.8:1.7:11.3. Scavenging activities of EPS-MS79 were 76% and 85% as measured by DPPH and 26% and 44% as measured by ABTS. The antiproliferative activities against colon (72% and 77%) and breast (43% and 56%) cancer cell lines. EPS-MS79 inhibited the growth of bacterial pathogens by up to 3 logs compared to the control. All EPS-MS79 solutions displayed shear thinning behavior. The storage and loss moduli of all EPS-MS79 solutions increased alongside with the frequency increase. Salt type and pH level impacted on the rheological properties of EPS-MS79. The EPS-MS79 could be a potent prebiotic promoting health and rheological properties of foods.",

keywords = "Anticancer, Antidiabetic, Apparent viscosity, Probiotic, Thixotropic",

author = "Mutamed Ayyash and Constantinos Stathopoulos and Basim Abu-Jdayil and Gennaro Esposito and Mohammad Baig and Turner, {Mark S.} and Baba, {Ahmad Salihin} and Vasso Apostolopoulos and Anas Al-Nabulsi and Tareq Osaili",

note = "Funding Information: This study was funded by the United Arab Emirates University (UAEU) via the UPAR project grant code G00003248 (31F135) . Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

year = "2020",

month = sep,

doi = "10.1016/j.lwt.2020.109741",

language = "English",

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AU - Ayyash, Mutamed

AU - Stathopoulos, Constantinos

AU - Abu-Jdayil, Basim

AU - Esposito, Gennaro

AU - Baig, Mohammad

AU - Turner, Mark S.

AU - Baba, Ahmad Salihin

AU - Apostolopoulos, Vasso

AU - Al-Nabulsi, Anas

AU - Osaili, Tareq

PY - 2020/9

Y1 - 2020/9

N2 - Microbial exopolysaccharide (EPS) is of interest due to their applications in food processing and potential health benefits following consumption. The objectives of this study were to: 1) isolate, purify and characterize EPS produced by Enterococcus faecium MS79 (EPS-MS79); 2) investigate bioactive properties of EPS-MS79; and 3) investigate the rheological properties of the EPS-MS79 as influenced by salt type and pH level. The average molecular weight of the EPS-MS79 was 8.3 × 105 Da and it is composed of the three monosaccharides arabinose, mannose, and glucose in a molar ratio of 0.8:1.7:11.3. Scavenging activities of EPS-MS79 were 76% and 85% as measured by DPPH and 26% and 44% as measured by ABTS. The antiproliferative activities against colon (72% and 77%) and breast (43% and 56%) cancer cell lines. EPS-MS79 inhibited the growth of bacterial pathogens by up to 3 logs compared to the control. All EPS-MS79 solutions displayed shear thinning behavior. The storage and loss moduli of all EPS-MS79 solutions increased alongside with the frequency increase. Salt type and pH level impacted on the rheological properties of EPS-MS79. The EPS-MS79 could be a potent prebiotic promoting health and rheological properties of foods.

AB - Microbial exopolysaccharide (EPS) is of interest due to their applications in food processing and potential health benefits following consumption. The objectives of this study were to: 1) isolate, purify and characterize EPS produced by Enterococcus faecium MS79 (EPS-MS79); 2) investigate bioactive properties of EPS-MS79; and 3) investigate the rheological properties of the EPS-MS79 as influenced by salt type and pH level. The average molecular weight of the EPS-MS79 was 8.3 × 105 Da and it is composed of the three monosaccharides arabinose, mannose, and glucose in a molar ratio of 0.8:1.7:11.3. Scavenging activities of EPS-MS79 were 76% and 85% as measured by DPPH and 26% and 44% as measured by ABTS. The antiproliferative activities against colon (72% and 77%) and breast (43% and 56%) cancer cell lines. EPS-MS79 inhibited the growth of bacterial pathogens by up to 3 logs compared to the control. All EPS-MS79 solutions displayed shear thinning behavior. The storage and loss moduli of all EPS-MS79 solutions increased alongside with the frequency increase. Salt type and pH level impacted on the rheological properties of EPS-MS79. The EPS-MS79 could be a potent prebiotic promoting health and rheological properties of foods.

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Exopolysaccharide produced by potential probiotic Enterococcus faecium MS79: Characterization, bioactivities and rheological properties influenced by salt and pH

Abstract

Keywords

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