Abstract
The present study was conducted to evaluate the role of different levels of Methyl jasmonate (MeJA) i.e. control, 1, 2.5 and 5 mM to minimize pre-harvest sprouting in rice. The results suggested that MeJA (5 mM) significantly reduced germination percentage, germination energy, germination index, shoot height, root length and seedling biomass in comparison with the control treatment. This reduction was more obvious in cultivar Zhu Liang You 06 (ZY) than in Qian You No.1 (QY). The activity of antioxidative enzymes i.e. catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) contents were significantly increased after treating with 1 mM and 2.5 mM concentrations of MeJA. However, higher concentration of MeJA (5 mM) significantly reduced the activities of antioxidant enzymes, seedling biomass and increased ROS generation. The a-amylase activity was significantly decreased with seed soaking in 5 mM of MeJA as compared to the control. Gene expression analysis suggested a significant up regulation of root Amy2A and Amy3A mRNA in comparison with the shoot, by seed soaking in MeJA. Higher concentration of MeJA (5 mM) improved resistance to pre-harvest sprouting in both rice cultivars. However, little cell damage and higher ROS accumulation were also observed.
| Original language | English |
|---|---|
| Pages (from-to) | 60-71 |
| Number of pages | 12 |
| Journal | Pakistan Journal of Life and Social Sciences |
| Volume | 15 |
| Issue number | 1 |
| Publication status | Published - 2017 |
| Externally published | Yes |
Keywords
- Inhibitory mechanism
- Methyl jasmonate
- Pre-harvest sprouting
- ROS
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology
- General Environmental Science
- General Social Sciences
- General Agricultural and Biological Sciences