TY - JOUR
T1 - FISH for mapping single copy genes.
AU - Raudsepp, Terje
AU - Chowdhary, Bhanu P.
PY - 2008
Y1 - 2008
N2 - During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.
AB - During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.
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U2 - 10.1007/978-1-59745-581-7_3
DO - 10.1007/978-1-59745-581-7_3
M3 - Article
C2 - 18629659
AN - SCOPUS:48849112044
SN - 1064-3745
VL - 422
SP - 31
EP - 49
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -