FISH mapping of seven cDNA sequences in the pig

R. Chaudhary, A. K. Winterø, M. Fredholm, B. P. Chowdhary

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7 Citations (Scopus)


Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28-q29, 7q2.6 and 5p11 respectively. The available human-pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL@). These clones mapped to SSC14q21, 5q2.4 and 14q22-q23 respectively. The remaining clone representing an EST mapped to 1p24-p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.

Original languageEnglish
Pages (from-to)545-549
Number of pages5
JournalChromosome Research
Issue number8
Publication statusPublished - 1997
Externally publishedYes


  • Fluorescence in situ hybridization
  • Pig
  • cDNA

ASJC Scopus subject areas

  • Genetics


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