FISH mapping of seven cDNA sequences in the pig

R. Chaudhary; A. K. Winterø; M. Fredholm; B. P. Chowdhary

doi:10.1023/A:1018445820059

FISH mapping of seven cDNA sequences in the pig

R. Chaudhary, A. K. Winterø, M. Fredholm, B. P. Chowdhary

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28-q29, 7q2.6 and 5p11 respectively. The available human-pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL@). These clones mapped to SSC14q21, 5q2.4 and 14q22-q23 respectively. The remaining clone representing an EST mapped to 1p24-p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.

Original language	English
Pages (from-to)	545-549
Number of pages	5
Journal	Chromosome Research
Volume	5
Issue number	8
DOIs	https://doi.org/10.1023/A:1018445820059
Publication status	Published - 1997
Externally published	Yes

Keywords

Fluorescence in situ hybridization
Pig
cDNA

ASJC Scopus subject areas

Genetics

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title = "FISH mapping of seven cDNA sequences in the pig",

abstract = "Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28-q29, 7q2.6 and 5p11 respectively. The available human-pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL@). These clones mapped to SSC14q21, 5q2.4 and 14q22-q23 respectively. The remaining clone representing an EST mapped to 1p24-p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.",

keywords = "Fluorescence in situ hybridization, Pig, cDNA",

author = "R. Chaudhary and Winter{\o}, {A. K.} and M. Fredholm and Chowdhary, {B. P.}",

note = "Funding Information: The project was financially supported by the Swedish Council for Forestry and Agricultural Research and the Danish Agricultural and Veterinary Research Council.",

year = "1997",

doi = "10.1023/A:1018445820059",

language = "English",

volume = "5",

pages = "545--549",

journal = "Chromosome Research",

issn = "0967-3849",

publisher = "Springer Netherlands",

number = "8",

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T1 - FISH mapping of seven cDNA sequences in the pig

AU - Chaudhary, R.

AU - Winterø, A. K.

AU - Fredholm, M.

AU - Chowdhary, B. P.

N1 - Funding Information: The project was financially supported by the Swedish Council for Forestry and Agricultural Research and the Danish Agricultural and Veterinary Research Council.

PY - 1997

Y1 - 1997

N2 - Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28-q29, 7q2.6 and 5p11 respectively. The available human-pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL@). These clones mapped to SSC14q21, 5q2.4 and 14q22-q23 respectively. The remaining clone representing an EST mapped to 1p24-p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.

AB - Fluorescence in situ hybridization (FISH) technique was applied to localize seven clones derived from a porcine (SSC) intestinal directionally cloned cDNA library. The size of the clones ranged from 1.1 to 1.3 kb. Three of the clones corresponded to histidyl-tRNA synthetase (HARS), immunoglobulin alpha (IGA) and lysozyme (LYZ) and mapped to SSC2q28-q29, 7q2.6 and 5p11 respectively. The available human-pig comparative painting data and sequence homology comparisons assisted in a tentative identification of the other three clones as glutathione-S-transferase (GST), glutathione-S-transferase mu (GSTM1) and immunoglobulin lambda gene cluster (IGL@). These clones mapped to SSC14q21, 5q2.4 and 14q22-q23 respectively. The remaining clone representing an EST mapped to 1p24-p25. These localizations contribute to the transcript map in pig and are significant as comparative markers. Difficulties associated with the mapping of small sequences using FISH are discussed.

KW - Fluorescence in situ hybridization

KW - Pig

KW - cDNA

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DO - 10.1023/A:1018445820059

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SN - 0967-3849

VL - 5

SP - 545

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JO - Chromosome Research

JF - Chromosome Research

IS - 8

ER -

FISH mapping of seven cDNA sequences in the pig

Abstract

Keywords

ASJC Scopus subject areas

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