Fragments of prosomatostatin isolated from a human pancreatic tumour

J. M. Conlon; D. M. McCarthy

doi:10.1016/0303-7207(84)90148-5

Fragments of prosomatostatin isolated from a human pancreatic tumour

J. M. Conlon, D. M. McCarthy

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Six peptides from an extract of a human pancreatic tumour were purified to homogeneity by HPLC. Amino acid composition and chemical properties indicated probable structures the same as somatostatin-14, somatostatin-28 (1-12), [Met(O)⁸]somatostatin-28 (1-12), [Met(O)⁸]somatostatin-28 (1-10), somatostatin-25 (1-9) and [Met(O)³]somatostatin-25 (1-9). The structure of somatostatin-14 was confirmed by sequence analysis. The isolation of these peptides provides insight into the processing of prosomatostatin in the tumour cells.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/0303-7207(84)90148-5
Publication status	Published - Nov 1984
Externally published	Yes

Keywords

HPLC
limited proteolysis
methionine oxidation

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(84)90148-5

Cite this

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title = "Fragments of prosomatostatin isolated from a human pancreatic tumour",

abstract = "Six peptides from an extract of a human pancreatic tumour were purified to homogeneity by HPLC. Amino acid composition and chemical properties indicated probable structures the same as somatostatin-14, somatostatin-28 (1-12), [Met(O)8]somatostatin-28 (1-12), [Met(O)8]somatostatin-28 (1-10), somatostatin-25 (1-9) and [Met(O)3]somatostatin-25 (1-9). The structure of somatostatin-14 was confirmed by sequence analysis. The isolation of these peptides provides insight into the processing of prosomatostatin in the tumour cells.",

keywords = "HPLC, limited proteolysis, methionine oxidation",

author = "Conlon, {J. M.} and McCarthy, {D. M.}",

note = "Funding Information: The authors wish to thank the Stiftung Volkswagenwerk for financial support and Profes- sor Dr. N. Hilschmann, Max-Planck-Institut fur Experimentelle Medizin, for providing facilities for amino acid analysis.",

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T1 - Fragments of prosomatostatin isolated from a human pancreatic tumour

AU - Conlon, J. M.

AU - McCarthy, D. M.

N1 - Funding Information: The authors wish to thank the Stiftung Volkswagenwerk for financial support and Profes- sor Dr. N. Hilschmann, Max-Planck-Institut fur Experimentelle Medizin, for providing facilities for amino acid analysis.

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Y1 - 1984/11

N2 - Six peptides from an extract of a human pancreatic tumour were purified to homogeneity by HPLC. Amino acid composition and chemical properties indicated probable structures the same as somatostatin-14, somatostatin-28 (1-12), [Met(O)8]somatostatin-28 (1-12), [Met(O)8]somatostatin-28 (1-10), somatostatin-25 (1-9) and [Met(O)3]somatostatin-25 (1-9). The structure of somatostatin-14 was confirmed by sequence analysis. The isolation of these peptides provides insight into the processing of prosomatostatin in the tumour cells.

AB - Six peptides from an extract of a human pancreatic tumour were purified to homogeneity by HPLC. Amino acid composition and chemical properties indicated probable structures the same as somatostatin-14, somatostatin-28 (1-12), [Met(O)8]somatostatin-28 (1-12), [Met(O)8]somatostatin-28 (1-10), somatostatin-25 (1-9) and [Met(O)3]somatostatin-25 (1-9). The structure of somatostatin-14 was confirmed by sequence analysis. The isolation of these peptides provides insight into the processing of prosomatostatin in the tumour cells.

KW - HPLC

KW - limited proteolysis

KW - methionine oxidation

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Fragments of prosomatostatin isolated from a human pancreatic tumour

Abstract

Keywords

ASJC Scopus subject areas

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