TY - JOUR
T1 - Function and selectivity of bromodomains in anchoring chromatin-modifying complexes to promoter nucleosomes
AU - Hassan, Ahmed H.
AU - Prochasson, Philippe
AU - Neely, Kristen E.
AU - Galasinski, Scott C.
AU - Chandy, Mark
AU - Carrozza, Michael J.
AU - Workman, Jerry L.
N1 - Funding Information:
We thank C. Brown for generating the tra1-28 allele and L. Howe for advice in designing experiments. We thank F. Winston for providing ΔBr Spt7 plasmid and strain and Δgcn5Δspt7 double deletion strain. We thank B. Laurent for ΔBr Swi2/Snf2 plasmid (pIT BL-SNF 1-1554; Laurent et al., 1993 ). We are grateful to B. Cairns for providing α Swp61/Arp7 and other antibodies against SWI/SNF subunits. We also thank R. Utley for Gal4-VP16 purification. We are grateful to J. Cote, T. Owen-Hughes, A. Eberharter, P. Grant, and members of the Workman, Simpson, Reese, and Tan labs at Penn State University for many helpful comments during this study. This work was supported by a grant from NIGMS to J.L.W. J.L.W. is an Associate Investigator with the Howard Hughes Medical Institute.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - The functions of the SAGA and SWI/SNF complexes are interrelated and can form stable "epigenetic marks" on promoters in vivo. Here we show that stable promoter occupancy by SWI/SNF and SAGA in the absence of transcription activators requires the bromodomains of the Swi2/Snf2 and Gcn5 subunits, respectively, and nucleosome acetylation. This acetylation can be brought about by either the SAGA or NuA4 HAT complexes. The bromodomain in the Spt7 subunit of SAGA is dispensable for this activity but will anchor SAGA if it is swapped into Gcn5, indicating that specificity of bromodomain function is determined in part by the subunit it occupies. Thus, bromodomains within the catalytic subunits of SAGA and SWI/SNF anchor these complexes to acetylated promoter nucleosomes.
AB - The functions of the SAGA and SWI/SNF complexes are interrelated and can form stable "epigenetic marks" on promoters in vivo. Here we show that stable promoter occupancy by SWI/SNF and SAGA in the absence of transcription activators requires the bromodomains of the Swi2/Snf2 and Gcn5 subunits, respectively, and nucleosome acetylation. This acetylation can be brought about by either the SAGA or NuA4 HAT complexes. The bromodomain in the Spt7 subunit of SAGA is dispensable for this activity but will anchor SAGA if it is swapped into Gcn5, indicating that specificity of bromodomain function is determined in part by the subunit it occupies. Thus, bromodomains within the catalytic subunits of SAGA and SWI/SNF anchor these complexes to acetylated promoter nucleosomes.
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U2 - 10.1016/S0092-8674(02)01005-X
DO - 10.1016/S0092-8674(02)01005-X
M3 - Article
C2 - 12419247
AN - SCOPUS:0036847620
SN - 0092-8674
VL - 111
SP - 369
EP - 379
JO - Cell
JF - Cell
IS - 3
ER -