Functional Genomics of Symbiotic Nitrogen Fixation in Legumes with a Focus on Transcription Factors and Membrane Transporters

Senjuti Sinharoy, Igor S. Kryvoruchko, Catalina I. Pislariu, Manuel González-Guerrero, Vagner A. Benedito, Michael K. Udvardi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Transcription factors (TFs), transcription regulators (TR), and membrane transporters are major determinants of nodule development and function. TFs and TRs control genetic reprogramming, and transporters facilitate exchange of nutrients between plant and rhizobia among other things. Here, we describe the strategy of reverse genetic screening in Medicago truncatula for genes from these two functional classes. We employed well-established transcriptomic resources (Medicago gene expression atlas), a spatially resolved nodule transcriptomic data set, and the tobacco retrotransposon Tnt1 insertion mutant population for systematic selection and functional analysis of candidate genes. We also re-annotated 417 nodule-induced TF and TR genes according to IMGAG3.5v4, which is the latest release of the Medicago genome. In total, we have targeted 21 nodule-enhanced TF and TR genes, and 20 nodule-enhanced transporter genes for characterization via insertional mutagenesis. In this chapter, we emphasize the importance of TFs, TRs, and transport proteins for symbiotic nitrogen fixation (SNF) and discuss the use of the Medicago Tnt1 mutant population for studies of gene function.

Original languageEnglish
Title of host publicationBiological Nitrogen Fixation
PublisherWiley Blackwell
Number of pages14
ISBN (Electronic)9781119053095
ISBN (Print)9781118637043
Publication statusPublished - Jan 1 2015
Externally publishedYes


  • Medicago gene expression atlas
  • Reverse genetic studies
  • Tnt1 mutant
  • Transcription factors
  • Transcription regulators
  • Transporters

ASJC Scopus subject areas

  • General Immunology and Microbiology


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