Functional Metabolomics Describes the Yeast Biosynthetic Regulome

Michael Mülleder, Enrica Calvani, Mohammad Tauqeer Alam, Richard Kangda Wang, Florian Eckerstorfer, Aleksej Zelezniak, Markus Ralser

Research output: Contribution to journalArticlepeer-review

123 Citations (Scopus)


Genome-metabolism interactions enable cell growth. To probe the extent of these interactions and delineate their functional contributions, we quantified the Saccharomyces amino acid metabolome and its response to systematic gene deletion. Over one-third of coding genes, in particular those important for chromatin dynamics, translation, and transport, contribute to biosynthetic metabolism. Specific amino acid signatures characterize genes of similar function. This enabled us to exploit functional metabolomics to connect metabolic regulators to their effectors, as exemplified by TORC1, whose inhibition in exponentially growing cells is shown to match an interruption in endomembrane transport. Providing orthogonal information compared to physical and genetic interaction networks, metabolomic signatures cluster more than half of the so far uncharacterized yeast genes and provide functional annotation for them. A major part of coding genes is therefore participating in gene-metabolism interactions that expose the metabolism regulatory network and enable access to an underexplored space in gene function.

Original languageEnglish
Pages (from-to)553-565.e12
Issue number2
Publication statusPublished - Oct 6 2016
Externally publishedYes


  • amino acids
  • functional gene annotation
  • functional metabolomics
  • mass spectrometry
  • metabolism
  • target of rapamycin (TOR)
  • unknown gene function
  • vesicle mediated transport
  • yeast gene deletion collection

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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