La G17 et la G17-gly stimulent la proliferation des cellules LoVo par l'intermediaire du recepteur CCK B/gastrine

Translated title of the contribution: Gastrin-17 and G17-gly induce proliferation through activation of CCK B/gastrin receptor in LoVo cells

Pascal Artru, Samir Attoub, Sandrine Levasseur, Miguel J.M. Lewin, André Bado

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background and methods. - Recent studies suggest that glycine-extended gastrin (G17-gly) stimulates in vitro proliferation of the pancreatic cell line AR4-2J, through selective receptors distinct from the CCK-B/G-receptor mediating the effects of amidated gastrin (G17). The aims of our study were to examine the effects of G17 and G17-gly on the growth of the colorectal cancer cell line LoVo and to determine the receptor involved by using selective receptor-antagonist. Results. - Both G17 and G17-gly stimulated [3H]-thymidine incorporation in a concentration-dependent fashion. Maximal stimulation (153 ± 18% and 166 ± 17% of control, p < 0.01) was achieved with 10 nM G17 and 100 nM G17-gly, respectively. These stimulations were fully prevented by the presence of 10pM YM022, a G/CCK B receptor-antagonist, but unaffected by L364,718, a CCK A receptor-antagonist. Basal growth of LoVo cells was inhibited by YM022 and stimulated by L364,718. CCKA and G/CCK B receptors mRNA were detected in the cells. Gastrin immunoreactivity was detected in the cells (16 pM) and in the extracellular medium (4.5 pM). Conclusion. - Both G17 and G17-gly stimulate LoVo cells growth through the activation of a gastrin/CCK B receptor. The evidence for secreted gastrin and CCK A and B receptors mRNA may further suggest the existence of an autocrine loop involving a stimulatory gastrin/CCK B receptor.

Translated title of the contributionGastrin-17 and G17-gly induce proliferation through activation of CCK B/gastrin receptor in LoVo cells
Original languageFrench
Pages (from-to)607-612
Number of pages6
JournalGastroenterologie Clinique et Biologique
Volume22
Issue number6-7
Publication statusPublished - Jun 1998
Externally publishedYes

Keywords

  • Autocrine loop
  • G/CCK B receptor
  • G17-gly
  • Gastrin-17
  • LoVo
  • YM022

ASJC Scopus subject areas

  • Gastroenterology

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