TY - JOUR
T1 - GC-MS analysis of commercially available allium sativum and trigonella foenum-graecum essential oils and their antimicrobial activities
AU - Ashraf, Syed Amir
AU - Khan, Mushtaq Ahmad
AU - Awadelkareem, Amir Mahgoub
AU - Tajuddin, Shaikh
AU - Ahmad, Md Faruque
AU - Hussain, Talib
N1 - Publisher Copyright:
© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
PY - 2019
Y1 - 2019
N2 - Food preservation and safety is drawing more attention globally due to the increasing prevalence of food-borne diseases. The natural methods of food preservation are considered safer compared to methods using synthetic preservatives. The essential oils with natural preservative properties could be useful for food safety and preservation. The objective of this study was to analyze the chemical composition of commercially available Allium sativum and Trigonella foenum-graecum essential oils by gas chromatography-mass spectroscopy (GC-MS). The antimicrobial activities of Allium sativum and Trigonella foenum-graecum essential oils were determined by agar well diffusion technique. The GC-MS analysis of Garlic essential oil (GEO) revealed that, Allyl methyl trisulfide (13.10%), Di-allyl sulfide (9.47%) and Di-allyl tetrasulfide (4.38%) were the major components, while methanolic extract of Fenugreek essential oil (FEO) showed limonene (12.92%), Silane trimethylphenyl (10.71%), carvone (4.57%) and Trigolline (0.38%) as major components. The results of our study showed a significant antimicrobial activity of GEO and FEO against the tested microbial strains, which indicates the presence of broad-spectrum antimicrobial constituents in GEO and FEO. However, further studies are needed for individual bioactive components and safety aspects for their application in food preservation.
AB - Food preservation and safety is drawing more attention globally due to the increasing prevalence of food-borne diseases. The natural methods of food preservation are considered safer compared to methods using synthetic preservatives. The essential oils with natural preservative properties could be useful for food safety and preservation. The objective of this study was to analyze the chemical composition of commercially available Allium sativum and Trigonella foenum-graecum essential oils by gas chromatography-mass spectroscopy (GC-MS). The antimicrobial activities of Allium sativum and Trigonella foenum-graecum essential oils were determined by agar well diffusion technique. The GC-MS analysis of Garlic essential oil (GEO) revealed that, Allyl methyl trisulfide (13.10%), Di-allyl sulfide (9.47%) and Di-allyl tetrasulfide (4.38%) were the major components, while methanolic extract of Fenugreek essential oil (FEO) showed limonene (12.92%), Silane trimethylphenyl (10.71%), carvone (4.57%) and Trigolline (0.38%) as major components. The results of our study showed a significant antimicrobial activity of GEO and FEO against the tested microbial strains, which indicates the presence of broad-spectrum antimicrobial constituents in GEO and FEO. However, further studies are needed for individual bioactive components and safety aspects for their application in food preservation.
KW - Agar well assay
KW - Antimicrobial activity
KW - Bio-active components
KW - Essential oils
KW - GC-MS
UR - http://www.scopus.com/inward/record.url?scp=85079130906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079130906&partnerID=8YFLogxK
U2 - 10.22207/JPAM.13.4.69
DO - 10.22207/JPAM.13.4.69
M3 - Article
AN - SCOPUS:85079130906
SN - 0973-7510
VL - 13
SP - 2545
EP - 2552
JO - Journal of Pure and Applied Microbiology
JF - Journal of Pure and Applied Microbiology
IS - 4
ER -