TY - JOUR
T1 - Genotoxicity of structurally related copper and zinc containing Schiff base complexes
AU - Khabour, Omar F.
AU - Saleh, Na'Il
AU - Alzoubi, Karem H.
AU - Hisaindee, Soleiman
AU - Al-Fyad, Doaa
AU - Al-Kaabi, Leena
AU - Dodeen, Arwa
AU - Esmadi, Fatima T.
N1 - Funding Information:
This work was supported by the Deanship of Research in Jordan University of Science and Technology (grant no.: 46/ 2011; to O.F.K.) and the Deanship of Research in Yarmouk University (grant no.: 29/2009; to F.T.E.).
PY - 2013/10
Y1 - 2013/10
N2 - The utilization of Schiff bases in the industrial and pharmaceutical fields has led to an increase in their syntheses and evaluation of their biological activities. In this study, we described the synthesis and genotoxicity of two Schiff bases that share common platform in their construction, namely, naphthalene, and are complexed to either Cu(II) or Zn(II). The genotoxicity of these complexes was evaluated in cultured lymphocytes using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs), and in rats using the urine 8-OH-2-deoxyguanosine (8-OH-dG) assay. The results showed that the examined complexes are genotoxic, but with different degrees. The order of genotoxicity of the complexes at 10/mL was: Cu(L3)(NCS)(H2O)>Zn(L 3)(NCS)(H2O)>Cu(L2)(NCS)>Zn(L2)(NCS), where L2 and L3 are the conjugate bases of N-(8-quinolyl)napthaldimine and N-(anilinyl)napthaldimine, respectively. However, at the 1-g/mL concentration, only the Cu(L3)(NCS)(H2O) complex induced significant CAs, whereas at the 0.1-g/mL concentration, only Cu(L3)(NCS)(H2O) and Zn(L2)(NCS) complexes induced significant SCEs, compared to controls. In the urine 8-OH-dG assay, all complexes at 10mg/100g body weight (b.w.) were found to cause DNA damage with the following order: Cu(L3)(NCS)(H2O)>Zn(L2)(NCS) >Zn(L3)(NCS)(H2O)>Cu(L2)(NCS), whereas no significant DNA damage was observed in animals exposed to 1 and 0.1mg/100g b.w. (p>0.05). In conclusion, the two examined Schiff base complexes are found to induce DNA damage, but with different degrees.
AB - The utilization of Schiff bases in the industrial and pharmaceutical fields has led to an increase in their syntheses and evaluation of their biological activities. In this study, we described the synthesis and genotoxicity of two Schiff bases that share common platform in their construction, namely, naphthalene, and are complexed to either Cu(II) or Zn(II). The genotoxicity of these complexes was evaluated in cultured lymphocytes using sister chromatid exchanges (SCEs) and chromosomal aberrations (CAs), and in rats using the urine 8-OH-2-deoxyguanosine (8-OH-dG) assay. The results showed that the examined complexes are genotoxic, but with different degrees. The order of genotoxicity of the complexes at 10/mL was: Cu(L3)(NCS)(H2O)>Zn(L 3)(NCS)(H2O)>Cu(L2)(NCS)>Zn(L2)(NCS), where L2 and L3 are the conjugate bases of N-(8-quinolyl)napthaldimine and N-(anilinyl)napthaldimine, respectively. However, at the 1-g/mL concentration, only the Cu(L3)(NCS)(H2O) complex induced significant CAs, whereas at the 0.1-g/mL concentration, only Cu(L3)(NCS)(H2O) and Zn(L2)(NCS) complexes induced significant SCEs, compared to controls. In the urine 8-OH-dG assay, all complexes at 10mg/100g body weight (b.w.) were found to cause DNA damage with the following order: Cu(L3)(NCS)(H2O)>Zn(L2)(NCS) >Zn(L3)(NCS)(H2O)>Cu(L2)(NCS), whereas no significant DNA damage was observed in animals exposed to 1 and 0.1mg/100g b.w. (p>0.05). In conclusion, the two examined Schiff base complexes are found to induce DNA damage, but with different degrees.
KW - 8-OH-dG
KW - Chromosomal aberrations
KW - Copper
KW - DNA damage
KW - Schiff bases
KW - Sister chromatid exchange
KW - Zinc
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UR - http://www.scopus.com/inward/citedby.url?scp=84883389173&partnerID=8YFLogxK
U2 - 10.3109/01480545.2013.776577
DO - 10.3109/01480545.2013.776577
M3 - Article
C2 - 23528207
AN - SCOPUS:84883389173
SN - 0148-0545
VL - 36
SP - 435
EP - 442
JO - Drug and Chemical Toxicology
JF - Drug and Chemical Toxicology
IS - 4
ER -