Ghrelin stimulates insulin secretion from the pancreas of normal and diabetic rats

E. Adeghate, A. S. Ponery

Research output: Contribution to journalArticlepeer-review

168 Citations (Scopus)

Abstract

Ghrelin is a novel 28-amino acid gut-brain peptide, which was first isolated in the rat stomach. This study examined the effect of ghrelin on insulin secretion from the isolated pancreas of normal and diabetic rats. Diabetes was induced by a single dose of streptozotocin. Four weeks after the induction of diabetes, pancreatic tissue fragments of normal and diabetic rats were treated with different concentrations (10-12, 10-9 and 10-6 M) of ghrelin. Ghrelin evoked large and significant increases in insulin secretion from the pancreas of both normal and diabetic rats. In the pancreas of normal rats, diltiazem (calcium channel antagonist) or a combination of atropine (muscarinic cholinergic receptor antagonist), propranolol (β-adrenergic receptor antagonist) and yohimbine (α2-adrenergic receptor antagonist) significantly reduced the stimulatory effect of ghrelin on insulin secretion. Diltiazem and yohimbine failed to inhibit ghrelin-evoked insulin release in diabetic rat pancreas. Ghrelin-immunoreactivity cells was observed in 2.6% and 3.8% of the total cell population in the islet of Langerhans of normal and diabetic rats, respectively.

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalJournal of Neuroendocrinology
Volume14
Issue number7
DOIs
Publication statusPublished - 2002

Keywords

  • Atropine
  • Diabetes mellitus
  • Diltiazem
  • EGTA
  • Ghrelin
  • Insulin secretion
  • Pancreas
  • Propranolol
  • Radioimmunoassay
  • Rat
  • Signal transduction
  • Yohimbine

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Ghrelin stimulates insulin secretion from the pancreas of normal and diabetic rats'. Together they form a unique fingerprint.

Cite this