TY - JOUR
T1 - High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction
AU - Eliasson, Christina
AU - Kamal-Eldin, Afaf
AU - Andersson, Roger
AU - Åman, Per
N1 - Funding Information:
The authors thanks Jan Meyer (Svalöf Weibull AB, Sweden) and Sven-Erik Larsson (Hushållningssällskapet, Örebro, Sweden) for providing Swedish flaxseed samples, and Lars Forsén (Alternativ Förädling AB, Glanshammar, Sweden) for providing a pressed flaxseed cake. This research was financed by Formas (2002-1558).
PY - 2003/9/19
Y1 - 2003/9/19
N2 - A HPLC method was developed for the analysis of secoisolariciresinol diglucoside (SDG) and hydroxycinnamic acid glucosides in milled defatted flaxseed flour. Direct extraction by 1 M NaOH for 1 h at 20°C resulted in a higher yield than that obtained by hydrolysis of alcoholic extracts. An internal standard, o-coumaric acid, was used and the method was found to be easy, fast, and with good repeatability. On dry matter basis, different samples of flaxseeds varied considerably in their content of (+)-SDG (11.9-25.9 mg/g), (-)-SDG (2.2-5.0 mg/g), p-coumaric acid glucoside (1.2-8.5 mg/g), and ferulic acid glucoside (1.6-5.0 mg/g).
AB - A HPLC method was developed for the analysis of secoisolariciresinol diglucoside (SDG) and hydroxycinnamic acid glucosides in milled defatted flaxseed flour. Direct extraction by 1 M NaOH for 1 h at 20°C resulted in a higher yield than that obtained by hydrolysis of alcoholic extracts. An internal standard, o-coumaric acid, was used and the method was found to be easy, fast, and with good repeatability. On dry matter basis, different samples of flaxseeds varied considerably in their content of (+)-SDG (11.9-25.9 mg/g), (-)-SDG (2.2-5.0 mg/g), p-coumaric acid glucoside (1.2-8.5 mg/g), and ferulic acid glucoside (1.6-5.0 mg/g).
KW - Coumaric acid glucoside
KW - Ferulic acid glucoside
KW - Glucosides
KW - Hydroxycinnamic acid glucosides
KW - Lignans
KW - Linum usitatissium
KW - Plant materials
KW - Secoisolariciresinol diglucoside
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U2 - 10.1016/S0021-9673(03)01136-1
DO - 10.1016/S0021-9673(03)01136-1
M3 - Article
C2 - 14521311
AN - SCOPUS:0142092527
SN - 0021-9673
VL - 1012
SP - 151
EP - 159
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -