High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay

M. A. Khan; J. B. Northwood; R. G.J. Loor; A. T.R. Tholen; E. Riera; M. Falcón; A. van Belkum; M. van Westreenen; J. P. Hays

doi:10.1111/j.1469-0691.2009.02898.x

High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay

M. A. Khan, J. B. Northwood, R. G.J. Loor, A. T.R. Tholen, E. Riera, M. Falcón, A. van Belkum, M. van Westreenen, J. P. Hays

Research output: Contribution to journal › Comment/debate › peer-review

21 Citations (Scopus)

Abstract

Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

Original language	English
Pages (from-to)	624-627
Number of pages	4
Journal	Clinical Microbiology and Infection
Volume	16
Issue number	6
DOIs	https://doi.org/10.1111/j.1469-0691.2009.02898.x
Publication status	Published - Jun 2010
Externally published	Yes

Keywords

Bacterial genotypes
Insertion sequences
Multilocus sequence typing
Transposons
Vancomycin-resistant Enterococci

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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Khan, M. A., Northwood, J. B., Loor, R. G. J., Tholen, A. T. R., Riera, E., Falcón, M., van Belkum, A., van Westreenen, M., & Hays, J. P. (2010). High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay. Clinical Microbiology and Infection, 16(6), 624-627. https://doi.org/10.1111/j.1469-0691.2009.02898.x

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abstract = "Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunci{\'o}n, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.",

keywords = "Bacterial genotypes, Insertion sequences, Multilocus sequence typing, Transposons, Vancomycin-resistant Enterococci",

author = "Khan, {M. A.} and Northwood, {J. B.} and Loor, {R. G.J.} and Tholen, {A. T.R.} and E. Riera and M. Falc{\'o}n and {van Belkum}, A. and {van Westreenen}, M. and Hays, {J. P.}",

note = "Funding Information: This work was financially supported by a European Union FP6 grant (DRESP2). The authors declare no conflict of interest.",

year = "2010",

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doi = "10.1111/j.1469-0691.2009.02898.x",

language = "English",

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AU - Khan, M. A.

AU - Northwood, J. B.

AU - Loor, R. G.J.

AU - Tholen, A. T.R.

AU - Riera, E.

AU - Falcón, M.

AU - van Belkum, A.

AU - van Westreenen, M.

AU - Hays, J. P.

N1 - Funding Information: This work was financially supported by a European Union FP6 grant (DRESP2). The authors declare no conflict of interest.

PY - 2010/6

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N2 - Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

AB - Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

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KW - Insertion sequences

KW - Multilocus sequence typing

KW - Transposons

KW - Vancomycin-resistant Enterococci

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High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay

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Keywords

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