High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay

M. A. Khan; J. B. Northwood; R. G.J. Loor; A. T.R. Tholen; E. Riera; M. Falcón; A. van Belkum; M. van Westreenen; J. P. Hays

doi:10.1111/j.1469-0691.2009.02898.x

High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay

M. A. Khan
, J. B. Northwood
, R. G.J. Loor
, A. T.R. Tholen
, E. Riera
, M. Falcón
, A. van Belkum
, M. van Westreenen
, J. P. Hays

Research output: Contribution to journal › Comment/debate › peer-review

21 Citations (Scopus)

Abstract

Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

Original language	English
Pages (from-to)	624-627
Number of pages	4
Journal	Clinical Microbiology and Infection
Volume	16
Issue number	6
DOIs	https://doi.org/10.1111/j.1469-0691.2009.02898.x
Publication status	Published - Jun 2010
Externally published	Yes

Keywords

Bacterial genotypes
Insertion sequences
Multilocus sequence typing
Transposons
Vancomycin-resistant Enterococci

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1111/j.1469-0691.2009.02898.x

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Khan, M. A., Northwood, J. B., Loor, R. G. J., Tholen, A. T. R., Riera, E., Falcón, M., van Belkum, A., van Westreenen, M., & Hays, J. P. (2010). High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay. Clinical Microbiology and Infection, 16(6), 624-627. https://doi.org/10.1111/j.1469-0691.2009.02898.x

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title = "High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunci{\'o}n, Paraguay",

abstract = "Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunci{\'o}n, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93\% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97\% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.",

keywords = "Bacterial genotypes, Insertion sequences, Multilocus sequence typing, Transposons, Vancomycin-resistant Enterococci",

author = "Khan, \{M. A.\} and Northwood, \{J. B.\} and Loor, \{R. G.J.\} and Tholen, \{A. T.R.\} and E. Riera and M. Falc{\'o}n and \{van Belkum\}, A. and \{van Westreenen\}, M. and Hays, \{J. P.\}",

note = "Funding Information: This work was financially supported by a European Union FP6 grant (DRESP2). The authors declare no conflict of interest.",

year = "2010",

month = jun,

doi = "10.1111/j.1469-0691.2009.02898.x",

language = "English",

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pages = "624--627",

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AU - Khan, M. A.

AU - Northwood, J. B.

AU - Loor, R. G.J.

AU - Tholen, A. T.R.

AU - Riera, E.

AU - Falcón, M.

AU - van Belkum, A.

AU - van Westreenen, M.

AU - Hays, J. P.

N1 - Funding Information: This work was financially supported by a European Union FP6 grant (DRESP2). The authors declare no conflict of interest.

PY - 2010/6

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N2 - Forty infection-associated VanA-type vancomycin-resistant Enterococcus faecium (VRE) strains obtained from five collaborating hospitals in Asunción, Paraguay were investigated. Genotyping using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing revealed the presence of 17 cluster types and four STs, with 93% (37/40) of isolates comprising ST type 78. Other ST types included ST-132, ST-210 and one new ST type (ST-438). All but one isolate (ST-438) were associated with clonal complex 17 (CC17), and 97% of the total isolates carried the esp gene. Three Tn. 1546 variants were found, including a new lineage containing an IS. Efa5 insertion in an existing IS1251 element.

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KW - Bacterial genotypes

KW - Insertion sequences

KW - Multilocus sequence typing

KW - Transposons

KW - Vancomycin-resistant Enterococci

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High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay

Abstract

Keywords

ASJC Scopus subject areas

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