TY - JOUR
T1 - HIV-1 env glycoproteins from two series of primary isolates
T2 - Replication phenotype and immunogenicity
AU - Mustafa, Farah
AU - Richmond, Joan F.L.
AU - Fernandez-Larsson, Roberto
AU - Lu, Shan
AU - Fredriksson, Robert
AU - Fenyö, Eva Maria
AU - O'Connell, Maryellen
AU - Johnson, Eric
AU - Weng, Jayu
AU - Santoro, Joseph C.
AU - Robinson, Harriet L.
N1 - Funding Information:
We are indebted to Drs. J. Arthos and J. I. Mullins for design and provision of pJW4303, Dr. J. Sodroski for the provision of psvIIIenv; Drs. P. Earl and C. Broder for the provision of vCB-14; and the AIDS Repository (Rockville, MD) for the provision of T-cell lines and the plasmids pNL4-3 and pHXB-2. We thank Drs. J. Sullivan and M. Somasundaran for provision of PBMCs. This work was supported by Public Health Service Grant RO1 A1 34241.
PY - 1997/3/3
Y1 - 1997/3/3
N2 - Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monomeric (gp120) and oligomeric (gp140) forms of the Envs using Env-expressing plasmid DNAs for immunizations. The env regions of the patient isolates conferred a spectrum of replication kinetics and cytotropisms on the NL4-3/env recombinants. Both patient series included nonsyncytium-inducing viruses with no ability to grow on T-cell lines, and highly syncytium inducing viruses which grew well on T-cell lines. These differences in growth potential did not correlate with the ability of the DNA-expressed Envs to raise antibody in rabbits. Rather, the relative immunogenicity of the Envs was patient and form specific. The Envs from patient 5 raised higher titers of antibody than the Envs from patient 6. For each primary Env, the gp120 form of the Env raised higher titers of antibody than the gp140 form. Thus, structural features of Env that affect replication do not necessarily affect the ability to raise antibody.
AB - Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monomeric (gp120) and oligomeric (gp140) forms of the Envs using Env-expressing plasmid DNAs for immunizations. The env regions of the patient isolates conferred a spectrum of replication kinetics and cytotropisms on the NL4-3/env recombinants. Both patient series included nonsyncytium-inducing viruses with no ability to grow on T-cell lines, and highly syncytium inducing viruses which grew well on T-cell lines. These differences in growth potential did not correlate with the ability of the DNA-expressed Envs to raise antibody in rabbits. Rather, the relative immunogenicity of the Envs was patient and form specific. The Envs from patient 5 raised higher titers of antibody than the Envs from patient 6. For each primary Env, the gp120 form of the Env raised higher titers of antibody than the gp140 form. Thus, structural features of Env that affect replication do not necessarily affect the ability to raise antibody.
UR - http://www.scopus.com/inward/record.url?scp=0031550774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031550774&partnerID=8YFLogxK
U2 - 10.1006/viro.1997.8445
DO - 10.1006/viro.1997.8445
M3 - Article
C2 - 9123870
AN - SCOPUS:0031550774
SN - 0042-6822
VL - 229
SP - 269
EP - 278
JO - Virology
JF - Virology
IS - 1
ER -