Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

J. Michael Conlon; Agnes Sonnevend; Carlos Davidson; Anni Demandt; Thierry Jouenne

doi:10.1016/j.dci.2004.05.003

Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

J. Michael Conlon, Agnes Sonnevend, Carlos Davidson, Anni Demandt, Thierry Jouenne

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC ₅₀<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH₂) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

Original language	English
Pages (from-to)	83-90
Number of pages	8
Journal	Developmental and Comparative Immunology
Volume	29
Issue number	1
DOIs	https://doi.org/10.1016/j.dci.2004.05.003
Publication status	Published - 2005
Externally published	Yes

Keywords

Antimicrobial peptide
Brevinin-1
Frog skin
HC, concentration producing 50% hemolysis
MIC, minimum inhibitory concentration
Phylogeny
Ranatuerin-2
Temporin

ASJC Scopus subject areas

Immunology
Developmental Biology

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10.1016/j.dci.2004.05.003

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title = "Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora",

abstract = "Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.",

keywords = "Antimicrobial peptide, Brevinin-1, Frog skin, HC, concentration producing 50% hemolysis, MIC, minimum inhibitory concentration, Phylogeny, Ranatuerin-2, Temporin",

author = "Conlon, {J. Michael} and Agnes Sonnevend and Carlos Davidson and Anni Demandt and Thierry Jouenne",

note = "Funding Information: This work was supported by an Interdisciplinary Grant (03/12-8-03-01) and a Faculty Support Grant (NP/04/02) from the United Arab Emirates University. The authors thank Lowell Diller and Laura Burkholder, Department of Wildlife, Humboldt State University for help in collection of specimens.",

year = "2005",

doi = "10.1016/j.dci.2004.05.003",

language = "English",

volume = "29",

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journal = "Developmental and Comparative Immunology",

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T1 - Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

AU - Conlon, J. Michael

AU - Sonnevend, Agnes

AU - Davidson, Carlos

AU - Demandt, Anni

AU - Jouenne, Thierry

N1 - Funding Information: This work was supported by an Interdisciplinary Grant (03/12-8-03-01) and a Faculty Support Grant (NP/04/02) from the United Arab Emirates University. The authors thank Lowell Diller and Laura Burkholder, Department of Wildlife, Humboldt State University for help in collection of specimens.

PY - 2005

Y1 - 2005

N2 - Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

AB - Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

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KW - Frog skin

KW - HC, concentration producing 50% hemolysis

KW - MIC, minimum inhibitory concentration

KW - Phylogeny

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KW - Temporin

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JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 1

ER -

Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

Abstract

Keywords

ASJC Scopus subject areas

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