HPLC analysis of K-48 concentration in plasma

H. Kalász; M. Y. Hasan; R. Sheen; K. Kuca; G. Petroianu; K. Ludányi; A. Gergely; K. Tekes

doi:10.1007/s00216-006-0490-6

HPLC analysis of K-48 concentration in plasma

H. Kalász, M. Y. Hasan, R. Sheen, K. Kuca, G. Petroianu, K. Ludányi, A. Gergely, K. Tekes

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

K-48 is a new oxime-type compound to be used as an enzyme reactivator in the treatment of exposure to organophosphorous compounds. Plasma concentration of K-48 can be determined using reversed-phase HPLC. Analysis using octyl silica stationary phase and ultraviolet-absorbance detection is fast and simple. K-48 displays a relatively high dose-normalized area under the curve as compared to pralidoxime, which might be beneficial for an antidote. After i.m. administration of 50 μmol K-48, the time course of the concentration can be approximated by a straight line between 15 and 120 min meaning the elimination follows zero-order kinetics.

Original language	English
Pages (from-to)	1062-1067
Number of pages	6
Journal	Analytical and Bioanalytical Chemistry
Volume	385
Issue number	6
DOIs	https://doi.org/10.1007/s00216-006-0490-6
Publication status	Published - Jul 2006

Keywords

Cholinesterase reactivator
HPLC
HPLC-MS
Serum concentration

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

Access to Document

10.1007/s00216-006-0490-6

Cite this

@article{3b5a25a01a6d4a88ba235a161b7c663e,

title = "HPLC analysis of K-48 concentration in plasma",

abstract = "K-48 is a new oxime-type compound to be used as an enzyme reactivator in the treatment of exposure to organophosphorous compounds. Plasma concentration of K-48 can be determined using reversed-phase HPLC. Analysis using octyl silica stationary phase and ultraviolet-absorbance detection is fast and simple. K-48 displays a relatively high dose-normalized area under the curve as compared to pralidoxime, which might be beneficial for an antidote. After i.m. administration of 50 μmol K-48, the time course of the concentration can be approximated by a straight line between 15 and 120 min meaning the elimination follows zero-order kinetics.",

keywords = "Cholinesterase reactivator, HPLC, HPLC-MS, Serum concentration",

author = "H. Kal{\'a}sz and Hasan, {M. Y.} and R. Sheen and K. Kuca and G. Petroianu and K. Lud{\'a}nyi and A. Gergely and K. Tekes",

note = "Funding Information: Acknowledgements This project was sponsored by a research grant of the Hungarian National Science and Research Fund (OTKA T049492). Advice from Dr. L.S. Ettre is appreciated.",

year = "2006",

month = jul,

doi = "10.1007/s00216-006-0490-6",

language = "English",

volume = "385",

pages = "1062--1067",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer Verlag",

number = "6",

}

TY - JOUR

T1 - HPLC analysis of K-48 concentration in plasma

AU - Kalász, H.

AU - Hasan, M. Y.

AU - Sheen, R.

AU - Kuca, K.

AU - Petroianu, G.

AU - Ludányi, K.

AU - Gergely, A.

AU - Tekes, K.

N1 - Funding Information: Acknowledgements This project was sponsored by a research grant of the Hungarian National Science and Research Fund (OTKA T049492). Advice from Dr. L.S. Ettre is appreciated.

PY - 2006/7

Y1 - 2006/7

N2 - K-48 is a new oxime-type compound to be used as an enzyme reactivator in the treatment of exposure to organophosphorous compounds. Plasma concentration of K-48 can be determined using reversed-phase HPLC. Analysis using octyl silica stationary phase and ultraviolet-absorbance detection is fast and simple. K-48 displays a relatively high dose-normalized area under the curve as compared to pralidoxime, which might be beneficial for an antidote. After i.m. administration of 50 μmol K-48, the time course of the concentration can be approximated by a straight line between 15 and 120 min meaning the elimination follows zero-order kinetics.

AB - K-48 is a new oxime-type compound to be used as an enzyme reactivator in the treatment of exposure to organophosphorous compounds. Plasma concentration of K-48 can be determined using reversed-phase HPLC. Analysis using octyl silica stationary phase and ultraviolet-absorbance detection is fast and simple. K-48 displays a relatively high dose-normalized area under the curve as compared to pralidoxime, which might be beneficial for an antidote. After i.m. administration of 50 μmol K-48, the time course of the concentration can be approximated by a straight line between 15 and 120 min meaning the elimination follows zero-order kinetics.

KW - Cholinesterase reactivator

KW - HPLC

KW - HPLC-MS

KW - Serum concentration

UR - http://www.scopus.com/inward/record.url?scp=33745593707&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745593707&partnerID=8YFLogxK

U2 - 10.1007/s00216-006-0490-6

DO - 10.1007/s00216-006-0490-6

M3 - Article

C2 - 16763789

AN - SCOPUS:33745593707

SN - 1618-2642

VL - 385

SP - 1062

EP - 1067

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 6

ER -

HPLC analysis of K-48 concentration in plasma

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this