HPLC analysis of sesaminol glucosides in sesame seeds

Ali A. Moazzami, Rolf E. Andersson, Afaf Kamal-Eldin

Research output: Contribution to journalArticlepeer-review

89 Citations (Scopus)


An HPLC method was developed and validated for the quantification of sesaminol triglucoside and a sesaminol diglucoside in sesame seeds. These two lignans were isolated, and their structures were characterized by mass and nuclear magnetic resonance spectroscopy. Defatted sesame flour was extracted first with 85% ethanol for 5 h followed by 70% ethanol for 10 h at room temperature using naringenin as internal standard. Analysis of 65 different samples of sesame seeds indicated that the content of sesaminol triglucoside ranged from 36 to 1560 mg/100 g of seed (mean 637 ± 312) and that of sesaminol diglucoside ranged from 0 to 493 mg/100 g of seed (mean 75 ± 95). No significant difference was found between sesaminol glucoside contents in black and white seeds.

Original languageEnglish
Pages (from-to)633-638
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number3
Publication statusPublished - Feb 8 2006
Externally publishedYes


  • HPLC analysis
  • Lignan
  • Sesame seed
  • Sesaminol diglucoside
  • Sesaminol triglucoside

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


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