HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds

Pernilla Johnsson; Afaf Kamal-Eldin; Lennart N. Lundgren; Per Åman

doi:10.1021/jf0005871

HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds

Pernilla Johnsson, Afaf Kamal-Eldin, Lennart N. Lundgren, Per Åman

Research output: Contribution to journal › Article › peer-review

181 Citations (Scopus)

Abstract

A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. The analytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using different column chromatographic methods and used, as external standard, for the calibration of the method and for quantification. The method was then applied to study the variation in SDG content in flaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG content varied between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g in whole flaxseeds.

Original language	English
Pages (from-to)	5216-5219
Number of pages	4
Journal	Journal of Agricultural and Food Chemistry
Volume	48
Issue number	11
DOIs	https://doi.org/10.1021/jf0005871
Publication status	Published - Dec 12 2000
Externally published	Yes

Keywords

Flaxseed
HPLC
Lignan
Secoisolarciresinol diglucoside

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

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10.1021/jf0005871

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title = "HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds",

abstract = "A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. The analytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using different column chromatographic methods and used, as external standard, for the calibration of the method and for quantification. The method was then applied to study the variation in SDG content in flaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG content varied between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g in whole flaxseeds.",

keywords = "Flaxseed, HPLC, Lignan, Secoisolarciresinol diglucoside",

author = "Pernilla Johnsson and Afaf Kamal-Eldin and Lundgren, {Lennart N.} and Per {\AA}man",

year = "2000",

month = dec,

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doi = "10.1021/jf0005871",

language = "English",

volume = "48",

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journal = "Journal of Agricultural and Food Chemistry",

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T1 - HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds

AU - Johnsson, Pernilla

AU - Kamal-Eldin, Afaf

AU - Lundgren, Lennart N.

AU - Åman, Per

PY - 2000/12/12

Y1 - 2000/12/12

N2 - A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. The analytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using different column chromatographic methods and used, as external standard, for the calibration of the method and for quantification. The method was then applied to study the variation in SDG content in flaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG content varied between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g in whole flaxseeds.

AB - A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. The analytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using different column chromatographic methods and used, as external standard, for the calibration of the method and for quantification. The method was then applied to study the variation in SDG content in flaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG content varied between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g in whole flaxseeds.

KW - Flaxseed

KW - HPLC

KW - Lignan

KW - Secoisolarciresinol diglucoside

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ER -

HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds

Abstract

Keywords

ASJC Scopus subject areas

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