HPLC method for analysis of secoisolariciresinol diglucoside in flaxseeds

Pernilla Johnsson, Afaf Kamal-Eldin, Lennart N. Lundgren, Per Åman

Research output: Contribution to journalArticlepeer-review

184 Citations (Scopus)


A method was developed for the analysis of secoisolariciresinol diglucoside (SDG) in flaxseeds. The analytical method involves extraction of defatted flaxseed flour with dioxane/ethanol, aqueous base-hydrolysis, solid-phase purification of an SDG-containing fraction, and quantitative analysis by high-performance liquid chromatography (HPLC). Pure SDG was isolated from flaxseed using different column chromatographic methods and used, as external standard, for the calibration of the method and for quantification. The method was then applied to study the variation in SDG content in flaxseeds from 14 cultivars grown in Sweden and 15 cultivars grown in Denmark. The SDG content varied between 11.7 and 24.1 mg/g in defatted flaxseed flour and between 6.1 and 13.3 mg/g in whole flaxseeds.

Original languageEnglish
Pages (from-to)5216-5219
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Issue number11
Publication statusPublished - 2000
Externally publishedYes


  • Flaxseed
  • HPLC
  • Lignan
  • Secoisolarciresinol diglucoside

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


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