Human and porcine immunoreactive gastric inhibitory polypeptides (IR-GIP) are not identical

A. J. Bacarese-Hamilton, T. E. Adrian, S. R. Bloom

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Immunoreactive gastric inhibitory polypeptide (IR-GIP) from human and porcine intestine was quantified by radioimmunoassay and the molecular forms characterised by gel permeation and reverse-phase high pressure liquid chromatography (HPLC). Gel filtration revealed two major immunoreactive peaks corresponding to the previously described 5-kDa and 8-kDa molecular forms, which appeared similar in both species. Isocratic reverse-phase HPLC revealed that the major immunoreactive GIP peak (5-kDa) in the human tissue eluted earlier than the corresponding porcine molecular form, indicating the latter to be less hydrophobic. These findings suggest significant species differences between human and porcine GIP.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalFEBS Letters
Issue number1
Publication statusPublished - Mar 12 1984
Externally publishedYes


  • Gastric inhibitory polypeptide
  • Gel filtration
  • Glucose-dependent insulinotropic peptide
  • Immunoreactive GIP
  • Molecular form
  • Reverse-phase HPLC
  • Species difference

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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