TY - JOUR
T1 - Human distribution and release of a putative new gut hormone, peptide YY
AU - Adrian, T. E.
AU - Ferri, G. L.
AU - Bacarese-Hamilton, A. J.
AU - Fuessl, H. S.
AU - Polak, J. M.
AU - Bloom, S. R.
N1 - Funding Information:
Received December 7, 1984. Accepted May 8. 1985. Address requests for reprints to: Professor S. R. Bloom, Department of Medicine, Hammersmith Hospital, DuCane Road, London W12 OHS, United Kingdom. This work was supported by the Medical Research Council and by a research contract from the Italian Cons&ho Nazionale delle Ricerche. H. S. Fuessl is the recipient of a Deutsche Forschungsge-meinschaft training fellowship. The authors thank Dr. K. Tatemoto and Professor V. Mutt of the Karolinska Institute for their gift of PYY and tryptic fragments, Dr. R. E. Chance of Lilly Research Laboratories for his gift of HPP and BPP, Professor J. Kimmel, Kansas, for his gift of APP. and Professor Yanaihara for his gift of synthetic PYY fragments. 0 1985 by the American Gastroenterological Association 0016-5085/85/$3.30
PY - 1985/11
Y1 - 1985/11
N2 - A radioimmunoassay has been developed for the new intestinal hormonal peptide tyrosine tyrosine [peptide YY (PYY)]. Peptide YY concentrations were measured in separated layers of the human gastrointestinal tract, where PYY was found exclusively in the mucosal epithelium which contained the endocrine cells. Peptide YY was found throughout the small intestine, in very low concentrations (5 pmol/g) in duodenum (6 pmol/g) and jejunum (5 pmpl/g), but in higher concentrations in the terminal ileum (84 pmol/g). High concentrations were found throughout the colon (ascending 82 pmol/g, sigmoid 196 pmol/g), being maximum in the rectum (480 pmol/g). The major molecular form of PYY-like immunoreactivity in human intestine appeared to be identical to pure porcine hormone, both as judged by gel permeation chromatography and by reversephase high-pressure liquid chromatography. Basal plasma concentrations of PYY were low but rose in response to food, remaining elevated for several hours postprandially. The known potent biologic actions of PYY, its high concentrations in gut endocrine cells, and its release into the circulation after a normal meal suggest that this peptide may function physiologically as a circulating gut hormone.
AB - A radioimmunoassay has been developed for the new intestinal hormonal peptide tyrosine tyrosine [peptide YY (PYY)]. Peptide YY concentrations were measured in separated layers of the human gastrointestinal tract, where PYY was found exclusively in the mucosal epithelium which contained the endocrine cells. Peptide YY was found throughout the small intestine, in very low concentrations (5 pmol/g) in duodenum (6 pmol/g) and jejunum (5 pmpl/g), but in higher concentrations in the terminal ileum (84 pmol/g). High concentrations were found throughout the colon (ascending 82 pmol/g, sigmoid 196 pmol/g), being maximum in the rectum (480 pmol/g). The major molecular form of PYY-like immunoreactivity in human intestine appeared to be identical to pure porcine hormone, both as judged by gel permeation chromatography and by reversephase high-pressure liquid chromatography. Basal plasma concentrations of PYY were low but rose in response to food, remaining elevated for several hours postprandially. The known potent biologic actions of PYY, its high concentrations in gut endocrine cells, and its release into the circulation after a normal meal suggest that this peptide may function physiologically as a circulating gut hormone.
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U2 - 10.1016/0016-5085(85)90211-2
DO - 10.1016/0016-5085(85)90211-2
M3 - Article
C2 - 3840109
AN - SCOPUS:0022398837
SN - 0016-5085
VL - 89
SP - 1070
EP - 1077
JO - Gastroenterology
JF - Gastroenterology
IS - 5
ER -