TY - JOUR
T1 - Identification and characterization of a cytosolic 30 kDa histamine stimulated phosphoprotein in parietal cell cytosol
AU - Oddsdottir, Margret
AU - Goldenring, James R.
AU - Adrian, Thomas E.
AU - Zdon, Michael J.
AU - Zucker, Karl A.
AU - Modlin, Irvin M.
PY - 1988/7/29
Y1 - 1988/7/29
N2 - Histamine stimulated acid secretion is mediated by an increase in intracellular cAMP. Cytosolic protein phosphorylation stimulated by histamine was investigated in isolated rabbit parietal cells. Histamine stimulated the phosphorylation of a 30 kDa phosphoprotein with an isoelectric point of 5.6. Cimetidine completely inhibited histamine-stimulated pp30 phosphorylation. However, omeprazole had no effect on the phosphorylation of pp30. Forskolin and 8-bromo-cAMP also stimulated the phosphorylation of pp30. The results suggest that pp30 is a histamine-stimulated, cAMP-dependently phosphorylated protein substrate in parietal cell cytosol.
AB - Histamine stimulated acid secretion is mediated by an increase in intracellular cAMP. Cytosolic protein phosphorylation stimulated by histamine was investigated in isolated rabbit parietal cells. Histamine stimulated the phosphorylation of a 30 kDa phosphoprotein with an isoelectric point of 5.6. Cimetidine completely inhibited histamine-stimulated pp30 phosphorylation. However, omeprazole had no effect on the phosphorylation of pp30. Forskolin and 8-bromo-cAMP also stimulated the phosphorylation of pp30. The results suggest that pp30 is a histamine-stimulated, cAMP-dependently phosphorylated protein substrate in parietal cell cytosol.
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U2 - 10.1016/0006-291X(88)90166-0
DO - 10.1016/0006-291X(88)90166-0
M3 - Article
C2 - 2840899
AN - SCOPUS:0023803079
SN - 0006-291X
VL - 154
SP - 489
EP - 496
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -