TY - JOUR
T1 - Identification and characterization of a novel gastric peptide hormone
T2 - The motilin-related peptide
AU - Tomasetto, Catherine
AU - Karam, Sherif M.
AU - Ribieras, Stephane
AU - Masson, Régis
AU - Lefebvre, Olivier
AU - Staub, Adrien
AU - Alexander, Glory
AU - Chenard, Marie Pierre
AU - Rio, Marie Christine
N1 - Funding Information:
Supported by funds from the Institut National de la Santé et de la Recherche Médicale, the Centre National de la Recherche Scientifique, the Hôpital Universitaire de Strasbourg, the Association pour la Recherche sur le Cancer, the Ligue Nationale Française contre le Cancer and the Comités du Haut-Rhin et du Bas-Rhin, and the Fondation de France and by grant MA038 from Kuwait University (to S.M.K.). Régis Masson was a recipient of an Association pour la Recherche sur le Cancer fellowship, and Stephane Ribieras was a recipient of a Ligue Nationale Française contre le Cancer fellowship.
PY - 2000
Y1 - 2000
N2 - Background and Aims: This study looked for new proteins with expression restricted to the gastric epithelium that may provide insight to the differentiation and function of the gastric unit. Methods: A novel complementary DNA was isolated and sequenced, and its expression was examined in mouse tissues at both messenger RNA and protein levels. Subcellular localization was studied using immunoelectron microscopy. The posttraductional processing of the protein was analyzed in vitro by protein microsequencing and in vivo by Western blotting. Results: We identified a novel protein that is mainly expressed by the secretory granules of the stomach enteroendocrine cells. This protein has sequence similarity with prepromotilin, the precursor of the motilin hormone and the motilin-associated peptide. As for the prepromotilin, a posttraductional maturation leads to a secreted peptide that is further cleaved at a dibasic site and gives rise to the motilin-related peptide (MTLRP) and MTLRP-associated peptide. Conclusions: We have identified and characterized a novel gene encoding the preproMTLRP protein. MTLRP presents similarity to motilin and is specifically expressed by enteroendocrine cells of the stomach and therefore represents a novel hormone.
AB - Background and Aims: This study looked for new proteins with expression restricted to the gastric epithelium that may provide insight to the differentiation and function of the gastric unit. Methods: A novel complementary DNA was isolated and sequenced, and its expression was examined in mouse tissues at both messenger RNA and protein levels. Subcellular localization was studied using immunoelectron microscopy. The posttraductional processing of the protein was analyzed in vitro by protein microsequencing and in vivo by Western blotting. Results: We identified a novel protein that is mainly expressed by the secretory granules of the stomach enteroendocrine cells. This protein has sequence similarity with prepromotilin, the precursor of the motilin hormone and the motilin-associated peptide. As for the prepromotilin, a posttraductional maturation leads to a secreted peptide that is further cleaved at a dibasic site and gives rise to the motilin-related peptide (MTLRP) and MTLRP-associated peptide. Conclusions: We have identified and characterized a novel gene encoding the preproMTLRP protein. MTLRP presents similarity to motilin and is specifically expressed by enteroendocrine cells of the stomach and therefore represents a novel hormone.
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U2 - 10.1053/gast.2000.9371
DO - 10.1053/gast.2000.9371
M3 - Article
C2 - 10930375
AN - SCOPUS:0033867587
SN - 0016-5085
VL - 119
SP - 395
EP - 405
JO - Gastroenterology
JF - Gastroenterology
IS - 2
M1 - 99524
ER -