Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein

Chung Yip Chan; Mohammad R. Salabat; Xian Zhong Ding; David L. Kelly; Mark S. Talamonti; Richard H. Bell; Thomas E. Adrian

doi:10.1016/j.bbrc.2005.02.037

Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein

Chung Yip Chan, Mohammad R. Salabat, Xian Zhong Ding, David L. Kelly, Mark S. Talamonti, Richard H. Bell, Thomas E. Adrian

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

Original language	English
Pages (from-to)	755-764
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	329
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2005.02.037
Publication status	Published - Apr 8 2005
Externally published	Yes

Keywords

12-O-Tetradecanoylphorbol-13-acetate
In silico analysis
Luciferase assay
Microarray
Novel protein
Protein kinase C
Real-time PCR
TTMP

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2005.02.037

Cite this

@article{72094db0b6ea47d79b950d8ac7bb5e08,

title = "Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein",

abstract = "12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.",

keywords = "12-O-Tetradecanoylphorbol-13-acetate, In silico analysis, Luciferase assay, Microarray, Novel protein, Protein kinase C, Real-time PCR, TTMP",

author = "Chan, {Chung Yip} and Salabat, {Mohammad R.} and Ding, {Xian Zhong} and Kelly, {David L.} and Talamonti, {Mark S.} and Bell, {Richard H.} and Adrian, {Thomas E.}",

note = "Funding Information: This work was supported by a grant from the National Cancer Institute (CA060553). C.-Y. Chan was supported by a fellowship from the National Medical Research Council and Tan Tock Seng Hospital, Singapore.",

year = "2005",

month = apr,

day = "8",

doi = "10.1016/j.bbrc.2005.02.037",

language = "English",

volume = "329",

pages = "755--764",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Identification and in silico characterization of a novel gene

T2 - TPA induced trans-membrane protein

AU - Chan, Chung Yip

AU - Salabat, Mohammad R.

AU - Ding, Xian Zhong

AU - Kelly, David L.

AU - Talamonti, Mark S.

AU - Bell, Richard H.

AU - Adrian, Thomas E.

N1 - Funding Information: This work was supported by a grant from the National Cancer Institute (CA060553). C.-Y. Chan was supported by a fellowship from the National Medical Research Council and Tan Tock Seng Hospital, Singapore.

PY - 2005/4/8

Y1 - 2005/4/8

N2 - 12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

AB - 12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

KW - 12-O-Tetradecanoylphorbol-13-acetate

KW - In silico analysis

KW - Luciferase assay

KW - Microarray

KW - Novel protein

KW - Protein kinase C

KW - Real-time PCR

KW - TTMP

UR - http://www.scopus.com/inward/record.url?scp=14644388201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644388201&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.02.037

DO - 10.1016/j.bbrc.2005.02.037

M3 - Article

C2 - 15737651

AN - SCOPUS:14644388201

SN - 0006-291X

VL - 329

SP - 755

EP - 764

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this