TY - JOUR
T1 - Identification of enteroinvasive Escherichia coli and Shigella strains in pediatric patients by an IpaC-specific enzyme-linked immunosorbent assay
AU - Pal, Tibor
AU - Al-Sweih, Noura Ahmed
AU - Herpay, Maria
AU - Chugh, Tulsi D.
PY - 1997/7
Y1 - 1997/7
N2 - A new method, a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) recognizing a secreted, invasion plasmid-coded protein antigen (IpaC), was used to identify enteroinvasive Escherichia coli and Shigella strains among colonies from 859 cultures of fecal samples from children in Kuwait. A total of 33.8% of the samples were diarrheal. By the immunoassay, enteroinvasive E. coli strains were identified from two diarrheal samples but from one of the samples from the children without diarrhea. These strains wee fully virulent and belonged to serogroup O28ac. In addition, 26 Shigella strains were also recognized by ELISA, while only 23 were isolated by routine biotyping and serotyping. For study showed that the IpaC-specific immunoassays is a simple and useful tool for identifying enteroinvasive strains. Furthermore, by report the first enteroinvasive E. coli isolates from Kuwait, the study indicates the presence of this group of pathogens as a potentials source of diarrhea in the region.
AB - A new method, a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) recognizing a secreted, invasion plasmid-coded protein antigen (IpaC), was used to identify enteroinvasive Escherichia coli and Shigella strains among colonies from 859 cultures of fecal samples from children in Kuwait. A total of 33.8% of the samples were diarrheal. By the immunoassay, enteroinvasive E. coli strains were identified from two diarrheal samples but from one of the samples from the children without diarrhea. These strains wee fully virulent and belonged to serogroup O28ac. In addition, 26 Shigella strains were also recognized by ELISA, while only 23 were isolated by routine biotyping and serotyping. For study showed that the IpaC-specific immunoassays is a simple and useful tool for identifying enteroinvasive strains. Furthermore, by report the first enteroinvasive E. coli isolates from Kuwait, the study indicates the presence of this group of pathogens as a potentials source of diarrhea in the region.
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U2 - 10.1128/jcm.35.7.1757-1760.1997
DO - 10.1128/jcm.35.7.1757-1760.1997
M3 - Article
C2 - 9196187
AN - SCOPUS:0031006303
SN - 0095-1137
VL - 35
SP - 1757
EP - 1760
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 7
ER -