Abstract
BALB/c mice were immunised with water extracts made from an Escherichia coli K-12 strain harbouring the shigella invasion plasmid, and hybridomas secreting antibodies specific to invasion plasmid-coded antigens were selected. On Western blots, antibodies produced by one of these clones (MAIC-1) recognised a protein of 43 kDa, which is the molecular mass of invasion plasmid coded antigen C (IpaC). When used in enzyme immunoassay against whole bacterial cells or against proteins secreted by actively growing bacteria, MAIC-1 clearly differentiated between invasive and non-invasive strains. Testing 123 enteroinvasive and 139 non-enteroinvasive strains the MAIC-1-based assay proved to be highly specific and sensitive in recognising enteroinvasive isolates. This test could be an inexpensive and rapid alternative to cumbersome virulence assays and a helpful technique in identifying Shigella or enteroinvasive Escherichia coli isolates.
Original language | English |
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Pages (from-to) | 111-117 |
Number of pages | 7 |
Journal | European Journal of Clinical Microbiology & Infectious Diseases |
Volume | 14 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 1995 |
Externally published | Yes |
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases