Identification of Shigella and enteroinvasive Escherichia coli strains by a virulence-specific, monoclonal antibody-based enzyme immunoassay

E. Floderus, T. Pál, K. Karlsson, A. A. Lindberg

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

BALB/c mice were immunised with water extracts made from an Escherichia coli K-12 strain harbouring the shigella invasion plasmid, and hybridomas secreting antibodies specific to invasion plasmid-coded antigens were selected. On Western blots, antibodies produced by one of these clones (MAIC-1) recognised a protein of 43 kDa, which is the molecular mass of invasion plasmid coded antigen C (IpaC). When used in enzyme immunoassay against whole bacterial cells or against proteins secreted by actively growing bacteria, MAIC-1 clearly differentiated between invasive and non-invasive strains. Testing 123 enteroinvasive and 139 non-enteroinvasive strains the MAIC-1-based assay proved to be highly specific and sensitive in recognising enteroinvasive isolates. This test could be an inexpensive and rapid alternative to cumbersome virulence assays and a helpful technique in identifying Shigella or enteroinvasive Escherichia coli isolates.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 1995
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Identification of Shigella and enteroinvasive Escherichia coli strains by a virulence-specific, monoclonal antibody-based enzyme immunoassay'. Together they form a unique fingerprint.

Cite this