Identifying a Gene Knockout Strategy Using a Hybrid of Simple Constrained Artificial Bee Colony Algorithm and Flux Balance Analysis to Enhance the Production of Succinate and Lactate in Escherichia Coli

Mei Kie Hon, Mohd Saberi Mohamad, Abdul Hakim Mohamed Salleh, Yee Wen Choon, Kauthar Mohd Daud, Muhammad Akmal Remli, Mohd Arfian Ismail, Sigeru Omatu, Richard O. Sinnott, Juan Manuel Corchado

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In recent years, metabolic engineering has gained central attention in numerous fields of science because of its capability to manipulate metabolic pathways in enhancing the expression of target phenotypes. Due to this, many computational approaches that perform genetic manipulation have been developed in the computational biology field. In metabolic engineering, conventional methods have been utilized to upgrade the generation of lactate and succinate in E. coli, although the yields produced are usually way below their theoretical maxima. To overcome the drawbacks of such conventional methods, development of hybrid algorithm is introduced to obtain an optimal solution by proposing a gene knockout strategy in E. coli which is able to improve the production of lactate and succinate. The objective function of the hybrid algorithm is optimized using a swarm intelligence optimization algorithm and a Simple Constrained Artificial Bee Colony (SCABC) algorithm. The results maximize the production of lactate and succinate by resembling the gene knockout in E. coli. The Flux Balance Analysis (FBA) is integrated in a hybrid algorithm to evaluate the growth rate of E. coli as well as the productions of lactate and succinate. This results in the identification of a gene knockout list that contributes to maximizing the production of lactate and succinate in E. coli.

Original languageEnglish
Pages (from-to)33-44
Number of pages12
JournalInterdisciplinary Sciences - Computational Life Sciences
Volume11
Issue number1
DOIs
Publication statusPublished - Mar 1 2019
Externally publishedYes

Keywords

  • Escherichia Coli
  • Flux Balance Analysis
  • Gene Knockout Strategies
  • Lactate
  • Simple Constrained Artificial Bee Colony
  • Succinate

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • Computer Science Applications
  • Health Informatics

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