Immobilization of Lipase on Metal-Organic frameworks for biodiesel production

Reem Shomal, Wei Du, Sulaiman Al-Zuhair

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


A comprehensive study on lipase immobilization on ZIF-67, ZIF-8, and HKUST-1 and their use in biodiesel production were performed. The highest adsorption capacity of 26.9 mg/g was achieved using ZIF-67 at 45 °C and an initial protein concentration of 0.6 mg/mL. Adsorption equilibrium data suggested that lipase adsorbed physically on ZIF-67 and ZIF-8 and chemically of HKUST-1. The data were best fitted with the Langmuir isotherm model for the three supports. Whereas, adsorption kinetics data were best fitted using Elovish's model for ZIF-67 and ZIF-8, and the pseudo-second-order model for HKUST-1. It was also found that the process was influenced by intraparticle and film diffusion. The prepared bio-catalyst was successfully used to catalyze biodiesel production in a co-solvent medium. The ZIF-8 and ZIF-67 showed better catalytic activity, achieving 88% and 90% conversion, whereas HKUST-1 showed better reusability due to the stronger chemical adoption. In addition, diffusion-reaction kinetics of biodiesel production using adsorbed lipase on ZIF-8 have been analysed. The investigation provided an insight into adsorption pathways and probable mechanisms involved and a better understanding of their application in biodiesel production. Mathematical diffusion-reaction modeling of lipase-MOFs biocatalyst used in biodiesel production, similar to the one presented in this work, is not found in literature.

Original languageEnglish
Article number107265
JournalJournal of Environmental Chemical Engineering
Issue number2
Publication statusPublished - Apr 2022


  • Adsorption
  • Biodiesel
  • Lipase
  • Metal-organic frameworks
  • Thermodynamic
  • Zeolitic Imidazolate frameworks

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Waste Management and Disposal
  • Pollution
  • Process Chemistry and Technology


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