Immunohistochemical identification of galanin and leucin-enkephalin in the porcine lacrimal gland

E. Adeghate; J. Singh

doi:10.1016/0143-4179(94)90109-0

Immunohistochemical identification of galanin and leucin-enkephalin in the porcine lacrimal gland

E. Adeghate, J. Singh

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The presence and distribution of galanin (GAL) and leucine-enkephalin (LEU-ENK) in the pig lacrimal gland was investigated using immunohistochemical techniques.Lacrimal gland segments obtained from the abattoir were fixed in paraformaldehyde-picric acid solution, cut into 40-50 μm thick sections and processed for immunohistochemistry using polyclonal antisera against GAL and LEU-ENK. GAL- and LEU-ENK-immunoreactive intrinsic multipolar neurons were observed in the interacinar and interlobular areas of the pig lacrimal gland. From these regions these intrinsic neurons send branches to the acinar and other tissues of the gland. Immunoreactivity was confined solely to the neural elements. These two potent biologically active neuropeptides may play important roles in the regulation of lacrimal fluid secretion.

Original language	English
Pages (from-to)	285-289
Number of pages	5
Journal	Neuropeptides
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/0143-4179(94)90109-0
Publication status	Published - Nov 1994

ASJC Scopus subject areas

Endocrinology
Neurology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

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10.1016/0143-4179(94)90109-0

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title = "Immunohistochemical identification of galanin and leucin-enkephalin in the porcine lacrimal gland",

abstract = "The presence and distribution of galanin (GAL) and leucine-enkephalin (LEU-ENK) in the pig lacrimal gland was investigated using immunohistochemical techniques.Lacrimal gland segments obtained from the abattoir were fixed in paraformaldehyde-picric acid solution, cut into 40-50 μm thick sections and processed for immunohistochemistry using polyclonal antisera against GAL and LEU-ENK. GAL- and LEU-ENK-immunoreactive intrinsic multipolar neurons were observed in the interacinar and interlobular areas of the pig lacrimal gland. From these regions these intrinsic neurons send branches to the acinar and other tissues of the gland. Immunoreactivity was confined solely to the neural elements. These two potent biologically active neuropeptides may play important roles in the regulation of lacrimal fluid secretion.",

author = "E. Adeghate and J. Singh",

note = "Funding Information: This work was supported by a grant from the Wellcome Trust (UK).",

year = "1994",

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doi = "10.1016/0143-4179(94)90109-0",

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journal = "Neuropeptides",

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T1 - Immunohistochemical identification of galanin and leucin-enkephalin in the porcine lacrimal gland

AU - Adeghate, E.

AU - Singh, J.

N1 - Funding Information: This work was supported by a grant from the Wellcome Trust (UK).

PY - 1994/11

Y1 - 1994/11

N2 - The presence and distribution of galanin (GAL) and leucine-enkephalin (LEU-ENK) in the pig lacrimal gland was investigated using immunohistochemical techniques.Lacrimal gland segments obtained from the abattoir were fixed in paraformaldehyde-picric acid solution, cut into 40-50 μm thick sections and processed for immunohistochemistry using polyclonal antisera against GAL and LEU-ENK. GAL- and LEU-ENK-immunoreactive intrinsic multipolar neurons were observed in the interacinar and interlobular areas of the pig lacrimal gland. From these regions these intrinsic neurons send branches to the acinar and other tissues of the gland. Immunoreactivity was confined solely to the neural elements. These two potent biologically active neuropeptides may play important roles in the regulation of lacrimal fluid secretion.

AB - The presence and distribution of galanin (GAL) and leucine-enkephalin (LEU-ENK) in the pig lacrimal gland was investigated using immunohistochemical techniques.Lacrimal gland segments obtained from the abattoir were fixed in paraformaldehyde-picric acid solution, cut into 40-50 μm thick sections and processed for immunohistochemistry using polyclonal antisera against GAL and LEU-ENK. GAL- and LEU-ENK-immunoreactive intrinsic multipolar neurons were observed in the interacinar and interlobular areas of the pig lacrimal gland. From these regions these intrinsic neurons send branches to the acinar and other tissues of the gland. Immunoreactivity was confined solely to the neural elements. These two potent biologically active neuropeptides may play important roles in the regulation of lacrimal fluid secretion.

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Immunohistochemical identification of galanin and leucin-enkephalin in the porcine lacrimal gland

Abstract

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