Immunolocalization of orexin-1 receptor the pancreas of normal and diabetic rats

E. Adeghate; R. S. Hameed

doi:10.1163/156939105774647402

Immunolocalization of orexin-1 receptor the pancreas of normal and diabetic rats

E. Adeghate, R. S. Hameed

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Orexins have been implicated in several biological functions, such as food and water intake. This study examined the pattern of distribution of orexin-1 receptor (OX₁R) in the pancreas of normal and diabetic rats. Diabetes mellitus (DM) was induced by streptozotocin (STZ). Four weeks after the induction of DM, the pancreases of normal and diabetic rats were processed for immunohistochemistry. OX₁R-positive cells were observed in the periphery of pancreatic islets of normal and in both the peripheral and central regions of the islet of diabetic rats. The number of OX₁R-positive cells in the islets increased significantly (p < 0.0001) after the onset of diabetes. Moreover, ganglion cells and the periductal and interlobular nerve fibres of the exocrine pancreas also expressed OX₁R. In conclusion, OX₁R may play a role in the initiation of increased food intake via pancreatic hormones.

Original language	English
Pages (from-to)	347-355
Number of pages	9
Journal	Biogenic Amines
Volume	19
Issue number	4-6
DOIs	https://doi.org/10.1163/156939105774647402
Publication status	Published - 2005

Keywords

Diabetes mellitus
Immunohistochemistry
Orexin receptors
Pancreas
Rat

ASJC Scopus subject areas

Neuroscience(all)
Pharmacology

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10.1163/156939105774647402

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title = "Immunolocalization of orexin-1 receptor the pancreas of normal and diabetic rats",

abstract = "Orexins have been implicated in several biological functions, such as food and water intake. This study examined the pattern of distribution of orexin-1 receptor (OX1R) in the pancreas of normal and diabetic rats. Diabetes mellitus (DM) was induced by streptozotocin (STZ). Four weeks after the induction of DM, the pancreases of normal and diabetic rats were processed for immunohistochemistry. OX1R-positive cells were observed in the periphery of pancreatic islets of normal and in both the peripheral and central regions of the islet of diabetic rats. The number of OX1R-positive cells in the islets increased significantly (p < 0.0001) after the onset of diabetes. Moreover, ganglion cells and the periductal and interlobular nerve fibres of the exocrine pancreas also expressed OX1R. In conclusion, OX1R may play a role in the initiation of increased food intake via pancreatic hormones.",

keywords = "Diabetes mellitus, Immunohistochemistry, Orexin receptors, Pancreas, Rat",

author = "E. Adeghate and Hameed, {R. S.}",

note = "Funding Information: This study was supported research grant from the Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, UAE.",

year = "2005",

doi = "10.1163/156939105774647402",

language = "English",

volume = "19",

pages = "347--355",

journal = "Biogenic Amines",

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TY - JOUR

T1 - Immunolocalization of orexin-1 receptor the pancreas of normal and diabetic rats

AU - Adeghate, E.

AU - Hameed, R. S.

N1 - Funding Information: This study was supported research grant from the Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, UAE.

PY - 2005

Y1 - 2005

N2 - Orexins have been implicated in several biological functions, such as food and water intake. This study examined the pattern of distribution of orexin-1 receptor (OX1R) in the pancreas of normal and diabetic rats. Diabetes mellitus (DM) was induced by streptozotocin (STZ). Four weeks after the induction of DM, the pancreases of normal and diabetic rats were processed for immunohistochemistry. OX1R-positive cells were observed in the periphery of pancreatic islets of normal and in both the peripheral and central regions of the islet of diabetic rats. The number of OX1R-positive cells in the islets increased significantly (p < 0.0001) after the onset of diabetes. Moreover, ganglion cells and the periductal and interlobular nerve fibres of the exocrine pancreas also expressed OX1R. In conclusion, OX1R may play a role in the initiation of increased food intake via pancreatic hormones.

AB - Orexins have been implicated in several biological functions, such as food and water intake. This study examined the pattern of distribution of orexin-1 receptor (OX1R) in the pancreas of normal and diabetic rats. Diabetes mellitus (DM) was induced by streptozotocin (STZ). Four weeks after the induction of DM, the pancreases of normal and diabetic rats were processed for immunohistochemistry. OX1R-positive cells were observed in the periphery of pancreatic islets of normal and in both the peripheral and central regions of the islet of diabetic rats. The number of OX1R-positive cells in the islets increased significantly (p < 0.0001) after the onset of diabetes. Moreover, ganglion cells and the periductal and interlobular nerve fibres of the exocrine pancreas also expressed OX1R. In conclusion, OX1R may play a role in the initiation of increased food intake via pancreatic hormones.

KW - Diabetes mellitus

KW - Immunohistochemistry

KW - Orexin receptors

KW - Pancreas

KW - Rat

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SN - 0168-8561

VL - 19

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EP - 355

JO - Biogenic Amines

JF - Biogenic Amines

IS - 4-6

ER -

Immunolocalization of orexin-1 receptor the pancreas of normal and diabetic rats

Abstract

Keywords

ASJC Scopus subject areas

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