Impact of lifelong sedentary behavior on mitochondrial function of mice skeletal muscle

Pedro A. Figueiredo, Scott K. Powers, Rita M. Ferreira, Francisco Amado, Hans J. Appell, José A. Duarte

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)


This study investigated the impact of lifelong sedentariness on skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y; old sedentary-OS; old active-OA). Young animals were sacrificed after 1 week of quarantine, and OS and OA groups were individually placed into standard cages and in cages with running wheels, respectively, until sacrifice (25 months). Body weights and hind-limb skeletal muscle wet weights were obtained from all groups. Mitochondrial respiratory functional measures (i.e., state 3 and 4 respiration, respiratory control ratio, and ratio of nanomoles of ADP phosphorylated by nanomoles of O2 consumed [ADP/O]) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups) were measured in isolated mitochondrial suspensions. Our results reveal that lifelong sedentary behavior has a negative impact on the age-related loss of skeletal muscle mass and on the isolated mitochondrial function of mixed skeletal muscle of mice, which is associated with an increased oxidative damage to mitochondrial biomolecules.

Original languageEnglish
Pages (from-to)927-939
Number of pages13
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number9
Publication statusPublished - Sept 2009
Externally publishedYes


  • Muscle aging
  • Oxidative stress
  • Running wheel
  • Sarcopenia
  • Voluntary activity

ASJC Scopus subject areas

  • General Medicine


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