Improved Extraction and Quality Characterization of Water-Soluble Polysaccharide from Gamma-Irradiated Lentinus edodes

Kashif Akram; Hafiz Muhammad Shahbaz; Gui Ran Kim; Umar Farooq; Joong Ho Kwon

doi:10.1111/1750-3841.13590

Improved Extraction and Quality Characterization of Water-Soluble Polysaccharide from Gamma-Irradiated Lentinus edodes

Kashif Akram, Hafiz Muhammad Shahbaz, Gui Ran Kim, Umar Farooq, Joong Ho Kwon

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Gamma irradiation was applied to the improved extraction of water-soluble polysaccharides (WSPs) from dried Lentinus edodes. Irradiation provided a dose-dependent increase in extraction yield (0 kGy, 2.01%; 7.5 kGy, 4.03%; 15 kGy, 7.17%) and purity (0 kGy, 78.8%; 7.5 kGy, 83.1%; 15 kGy, 85.6%) of the WSPs from hot-water extraction. The effect of irradiation was evident in the degraded microstructures and reduced molecular weights of the WSPs. However, nuclear magnetic resonance, Fourier-transform infrared, and X-ray diffraction spectroscopic analyses provided comparable structures of WSPs from nonirradiated and irradiated samples. UV–visible spectra showed a dose-dependent decline in intensity, but an improvement in thermal properties of the WSPs from the irradiated mushroom samples was observed.

Original language	English
Pages (from-to)	296-303
Number of pages	8
Journal	Journal of Food Science
Volume	82
Issue number	2
DOIs	https://doi.org/10.1111/1750-3841.13590
Publication status	Published - Feb 1 2017
Externally published	Yes

Keywords

extraction
gamma irradiation
Lentinus edodes
water-soluble polysaccharide

ASJC Scopus subject areas

Food Science

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10.1111/1750-3841.13590

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@article{47b5d740987845b080232b80737d8736,

title = "Improved Extraction and Quality Characterization of Water-Soluble Polysaccharide from Gamma-Irradiated Lentinus edodes",

abstract = "Gamma irradiation was applied to the improved extraction of water-soluble polysaccharides (WSPs) from dried Lentinus edodes. Irradiation provided a dose-dependent increase in extraction yield (0 kGy, 2.01%; 7.5 kGy, 4.03%; 15 kGy, 7.17%) and purity (0 kGy, 78.8%; 7.5 kGy, 83.1%; 15 kGy, 85.6%) of the WSPs from hot-water extraction. The effect of irradiation was evident in the degraded microstructures and reduced molecular weights of the WSPs. However, nuclear magnetic resonance, Fourier-transform infrared, and X-ray diffraction spectroscopic analyses provided comparable structures of WSPs from nonirradiated and irradiated samples. UV–visible spectra showed a dose-dependent decline in intensity, but an improvement in thermal properties of the WSPs from the irradiated mushroom samples was observed.",

keywords = "extraction, gamma irradiation, Lentinus edodes, water-soluble polysaccharide",

author = "Kashif Akram and Shahbaz, {Hafiz Muhammad} and Kim, {Gui Ran} and Umar Farooq and Kwon, {Joong Ho}",

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doi = "10.1111/1750-3841.13590",

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T1 - Improved Extraction and Quality Characterization of Water-Soluble Polysaccharide from Gamma-Irradiated Lentinus edodes

AU - Akram, Kashif

AU - Shahbaz, Hafiz Muhammad

AU - Kim, Gui Ran

AU - Farooq, Umar

AU - Kwon, Joong Ho

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Gamma irradiation was applied to the improved extraction of water-soluble polysaccharides (WSPs) from dried Lentinus edodes. Irradiation provided a dose-dependent increase in extraction yield (0 kGy, 2.01%; 7.5 kGy, 4.03%; 15 kGy, 7.17%) and purity (0 kGy, 78.8%; 7.5 kGy, 83.1%; 15 kGy, 85.6%) of the WSPs from hot-water extraction. The effect of irradiation was evident in the degraded microstructures and reduced molecular weights of the WSPs. However, nuclear magnetic resonance, Fourier-transform infrared, and X-ray diffraction spectroscopic analyses provided comparable structures of WSPs from nonirradiated and irradiated samples. UV–visible spectra showed a dose-dependent decline in intensity, but an improvement in thermal properties of the WSPs from the irradiated mushroom samples was observed.

AB - Gamma irradiation was applied to the improved extraction of water-soluble polysaccharides (WSPs) from dried Lentinus edodes. Irradiation provided a dose-dependent increase in extraction yield (0 kGy, 2.01%; 7.5 kGy, 4.03%; 15 kGy, 7.17%) and purity (0 kGy, 78.8%; 7.5 kGy, 83.1%; 15 kGy, 85.6%) of the WSPs from hot-water extraction. The effect of irradiation was evident in the degraded microstructures and reduced molecular weights of the WSPs. However, nuclear magnetic resonance, Fourier-transform infrared, and X-ray diffraction spectroscopic analyses provided comparable structures of WSPs from nonirradiated and irradiated samples. UV–visible spectra showed a dose-dependent decline in intensity, but an improvement in thermal properties of the WSPs from the irradiated mushroom samples was observed.

KW - extraction

KW - gamma irradiation

KW - Lentinus edodes

KW - water-soluble polysaccharide

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Improved Extraction and Quality Characterization of Water-Soluble Polysaccharide from Gamma-Irradiated Lentinus edodes

Abstract

Keywords

ASJC Scopus subject areas

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