Improved HPLC determination of acidic opines by phenylisothiocyanate derivatization and its application to marine animals

M. Emdadul Haque, Tomomi Fujisawa, Masamichi Yamamoto, Shinji Ohmori

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We present here a reliable and sensitive method for the determination of acidic opines such as meso-alanopine, β-alanopine, tauropine and strombine in biological samples. Interfering primary amino acids were eliminated by reaction with o-phthalaldehyde, and the derivatized compounds were passed through Sep-Pak Plus PS-1 cartridges. The acidic opines were recovered by flushing the cartridges with water, then determined by high performance liquid chromatography after a second derivatization with phenylisothiocyanate. All 4 acidic opines were detected within 30 min. This method ensured good separation and guaranteed almost full recovery of all acidic opines. This method was applied to analyze opines in marine animals and to test whether opines are metabolized in the livers of the rat and fish.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalActa Medica Okayama
Volume54
Issue number1
Publication statusPublished - Dec 1 2000
Externally publishedYes

Keywords

  • High-performance liquid chromatography determination
  • Marine animals
  • Opines
  • Phenylisothiocyanate
  • Sep-Pak cartridge

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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